Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China.
GongQing Institute of Science and Technology, Gongqingcheng 332020, Jiangxi, PR China.
Colloids Surf B Biointerfaces. 2022 Oct;218:112735. doi: 10.1016/j.colsurfb.2022.112735. Epub 2022 Aug 3.
The effective monitoring of nucleotides and their metabolites is critical for the prevention of various genetic metabolic diseases. In this aspect, surface-enhanced Raman scattering (SERS) as an ultrasensitive and nondestructive sensing techniques has spurted an attractive prospect. Henceforth, an effective SERS-based analysis of adenosine monophosphate (AMP) as well as adenine was realized here by utilizing an innovative kind of self-cleaning substrate constructed with graphitic carbon nitride and Au nanoflowers (g-CN@Au NFs). Taking advantages of the numerous nanotip-triggered "hotspot" regions of Au NFs as well as the excellent photocatalytic degradation capability of g-CN matrix, the hybrid substrate not only brought about a low limit of detection (LOD) of 5.01 × 10 M, but also yielded an attractive self-cleaning property. Particularly, these fascinating features of the proposed g-CN@Au NFs facilitated a recyclable monitoring of AMP and adenine in serum with reliable sensitivity and stability. The achieved wide linear range from 10 to 1 mg/mL together with the LOD values down to 10 mg/mL all envisioned that it is possible to monitor and investigate human metabolic processes by SERS protocol.
核苷酸及其代谢物的有效监测对预防各种遗传代谢疾病至关重要。在这方面，表面增强拉曼散射（SERS）作为一种超灵敏、非破坏性的传感技术，具有很大的吸引力。因此，通过利用一种由石墨相氮化碳和金纳米花（g-CN@Au NFs）构建的新型自清洁基底，实现了对腺苷一磷酸（AMP）和腺嘌呤的有效 SERS 分析。利用 Au NFs 的众多纳米尖端引发的“热点”区域以及 g-CN 基质优异的光催化降解能力，该混合基底不仅具有 5.01×10 M 的低检测限（LOD），而且还具有吸引人的自清洁性能。特别是，所提出的 g-CN@Au NFs 的这些迷人特性，为在血清中进行 AMP 和腺嘌呤的可回收监测提供了可靠的灵敏度和稳定性。所获得的从 10 到 1 mg/mL 的宽线性范围以及低至 10 mg/mL 的检测限值，都表明通过 SERS 方案有可能监测和研究人体代谢过程。
