Zheng Liqing, Li Ze, Wang Zhao, Shen Lixia, Liu Jianhua
Department of Pharmacology, College of pharmacy, Hebei North University, Zhangjiakou 075000, China.
Department of Pharmacology, College of pharmacy, Hebei North University, Zhangjiakou 075000, China; Hebei Key Laboratory of Neuropharmacology, Zhangjiakou 075000, China.
Photodiagnosis Photodyn Ther. 2022 Dec;40:103062. doi: 10.1016/j.pdpdt.2022.103062. Epub 2022 Aug 10.
To investigate the susceptibility of SH-SY5Y cells to DTPP-based photodynamic therapy (PDT) and the antagonistic effects of chrysophanol (Chr) on PDT.
PDT photocytotoxicity to cells was quantified and determined by exposing increasing concentrations of DTPP between 2.5 to 20 μg/mL to radiation with energy densities of 1.2-9.6 J/cm at 630-nm wavelength. Sodium azide (SA, NaN) and d-mannitol (DM) were employed to study the reaction type of PDT. The photodynamic stress after PDT was assessed by superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidative capacity (T-AOC) assays. The apoptosis pathway of SH-SY5Y cells after PDT was studied by the determination of JC-1 and caspase-9/Caspase-3 concentrations. MTT and double fluorescence staining assays were applied to study the effect of Chr on cell survival and apoptosis rate in PDT, respectively. PI was used to detect the effect of Chr on cell membrane integrity after DTPP-PDT treatment.
The dose-dependent killing effect of high DTPP concentrations and irradiation doses were identified. Cell apoptosis is mediated by a mitochondrial pathway with a total apoptosis rate of 33.8% at 10 μg/mL of DTPP after irradiation with 2.4 J/cm. Oxidative stress was produced by ROS in PDT and non-reversible cell oxidative damage appeared due to the cells' modulation of the oxidative stress balance during the PDT response. Chr had a- effect on ROS capture and an inhibitory effect on the PDT-induced destruction of cell membranes.
SH-SY5Y cells were susceptible to DTPP-PDT, resulting in a mitochondrial apoptosis pathway. There is an antagonistic effect of Chr on PDT in SH-SY5Y neuroblastoma cells.
研究SH-SY5Y细胞对基于二氢卟吩e6（DTPP）的光动力疗法（PDT）的敏感性以及大黄酚（Chr）对PDT的拮抗作用。
通过将2.5至20μg/mL浓度递增的DTPP暴露于波长630nm、能量密度为1.2 - 9.6J/cm²的辐射下，对细胞的PDT光细胞毒性进行定量和测定。使用叠氮化钠（SA，NaN₃）和d-甘露醇（DM）研究PDT的反应类型。通过超氧化物歧化酶（SOD）、丙二醛（MDA）和总抗氧化能力（T-AOC）测定评估PDT后的光动力应激。通过测定JC-1以及半胱天冬酶-9/半胱天冬酶-3浓度来研究PDT后SH-SY5Y细胞的凋亡途径。分别应用MTT和双荧光染色测定法研究Chr对PDT中细胞存活和凋亡率的影响。使用碘化丙啶（PI）检测Chr对DTPP-PDT处理后细胞膜完整性的影响。
确定了高DTPP浓度和照射剂量的剂量依赖性杀伤作用。细胞凋亡由线粒体途径介导，在2.4J/cm²照射后，10μg/mL的DTPP时总凋亡率为33.8%。PDT中活性氧（ROS）产生氧化应激，并且由于细胞在PDT反应过程中对氧化应激平衡的调节，出现了不可逆的细胞氧化损伤。Chr对ROS捕获有作用，并且对PDT诱导的细胞膜破坏有抑制作用。
SH-SY5Y细胞对DTPP-PDT敏感，导致线粒体凋亡途径。Chr对SH-SY5Y神经母细胞瘤细胞的PDT有拮抗作用。
