Huang Wen-Chung, Liou Chian-Jiun, Shen Szu-Chuan, Hu Sindy, Chao Jane C-J, Huang Chun-Hsun, Wu Shu-Ju
Graduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan City, Taiwan.
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan City, Taiwan.
Asian Pac J Allergy Immunol. 2022 Aug 22. doi: 10.12932/AP-200122-1309.
Olive (Olea europaea Linn) leaves contain a phenolic compound oleuropein (Ole) has antioxidant, anti-inflammatory, and immunomodulatory activities. However, whether Ole might be an effective treatment for atopic dermatitis (AD) remains unknown.
This study investigated the functional role of oleuropein in a 2,4-dinitrochlorobenzene-induced AD-like mouse model, with a focus on allergic inflammation.
We evaluated cytokine gene expression, COX-2 inflammatory protein production, and Th2 related cytokine regulation of mast cells and eosinophils that infiltrated AD-like skin lesions.
A topical application of Ole significantly reduced Th2-related cytokine gene expression (IL-4 and IL-5) and inflammatory COX-2 protein production in AD-like skin lesions. Additionally, Ole suppressed serum IgE levels. Furthermore, Ole effectively reduced ear swelling and epidermal and dermal thickening.
These results suggested that, mechanistically, Ole treatment improved allergic inflammation by blocking the Th2-driven inflammatory axis. In conclusion, our findings indicated that Ole showed promise in treating AD by regulating serum IgE and Th2 cytokine levels. Although the effects of Ole on AD in humans require clinical trials, our results provided insights into how AD treatments might be improved.
橄榄（油橄榄）叶含有一种酚类化合物橄榄苦苷（Ole），具有抗氧化、抗炎和免疫调节活性。然而，Ole是否可能是特应性皮炎（AD）的有效治疗方法仍不清楚。
本研究在2,4-二硝基氯苯诱导的AD样小鼠模型中研究了橄榄苦苷的功能作用，重点关注过敏性炎症。
我们评估了浸润AD样皮肤病变的肥大细胞和嗜酸性粒细胞的细胞因子基因表达、COX-2炎症蛋白产生以及Th2相关细胞因子调节。
局部应用Ole可显著降低AD样皮肤病变中Th2相关细胞因子基因表达（IL-4和IL-5）以及炎症性COX-2蛋白产生。此外，Ole可抑制血清IgE水平。此外，Ole有效减轻耳部肿胀以及表皮和真皮增厚。
这些结果表明，从机制上讲，Ole治疗通过阻断Th2驱动的炎症轴改善了过敏性炎症。总之，我们的研究结果表明，Ole通过调节血清IgE和Th2细胞因子水平在治疗AD方面显示出前景。尽管Ole对人类AD的影响需要进行临床试验，但我们的结果为如何改善AD治疗提供了见解。
