Xie Hai, Hu Qingqing, Qin Xiuting, Zhang Yali, Li Lu, Li Ji
College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, People's Republic of China.
College of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Dec 15;283:121740. doi: 10.1016/j.saa.2022.121740. Epub 2022 Aug 9.
The regulation of copper in the human body is important for the prevention of several diseases. Therefore, a rhodamine B-based chemosensor 1 that demonstrates substantial affinity and selectivity was synthesized for the fluorescence imaging of copper(II). In the presence of Cu, the chemosensor underwent a color change from colorless to amaranth that was visible to the naked eye, and the fluorescence intensity did not change when excess EDTA was added to the solution. Furthermore, strong fluorescence was observed at 575 nm. The limit of detection was determined as 12.1 nM. The absolute fluorescence quantum yield was as high as 77 % and the stoichiometry between 1 and Cu was determined to be 1:1 using a job plot. An analytical method was developed and successfully used to evaluate the sensor's ability for the fluorescence imaging of Cu in HeLa tumor cells.
人体中铜的调节对于预防多种疾病很重要。因此，合成了一种基于罗丹明B的化学传感器1，它对铜（II）具有显著的亲和力和选择性，用于铜（II）的荧光成像。在铜存在的情况下，化学传感器发生颜色变化，从无色变为紫红色，肉眼可见，并且当向溶液中加入过量乙二胺四乙酸（EDTA）时荧光强度不变。此外，在575nm处观察到强荧光。检测限确定为12.1 nM。绝对荧光量子产率高达77%，使用摩尔比法确定1与铜之间的化学计量比为1:1。开发了一种分析方法，并成功用于评估该传感器对HeLa肿瘤细胞中铜进行荧光成像的能力。
