Biochemistry and Biotechnology Laboratory, CSIR - Central Leather Research Institute, Adyar, Chennai 600 020, India; University of Madras, Chepauk, Chennai 600005, India.
Biochemistry and Biotechnology Laboratory, CSIR - Central Leather Research Institute, Adyar, Chennai 600 020, India.
Biomater Adv. 2022 Sep;140:213078. doi: 10.1016/j.bioadv.2022.213078. Epub 2022 Aug 8.
Prolonged inflammation, elevated matrix metalloproteinases, hypoxia, decreased vascularization, increased oxidative stress, and bacterial infection are typical signs of chronic non-healing diabetic wounds. Any agent that improves one or all factors could offer enhanced opportunities for better healing of diabetic wounds. In this study, a polyphenol (polydatin) incorporated collagen scaffold was prepared using a biocompatible crosslinker, oxidized pullulan (Col-OxP3-Po), to treat diabetic wounds. The scaffolds were characterized using SEM, FTIR, antioxidant activity, in vitro and in vivo wound healing assay, gene expression, and immunohistopathological studies. Polydatin incorporated scaffold exhibited 75 % antioxidant activity, hemostatic and erythrocyte adhesion properties. FTIR results proved the incorporation of polydatin in the Col-OxP3-Po scaffold. They were also non-toxic to the 3 T3 fibroblasts with a viability of 93 % and good cell attachment. In vivo, normal and diabetic wound healing studies showed that the Col-OxP3-Po scaffold treated group healed on days 16 and 21. The histological and immunohistochemistry analyses of the granulation tissues showed improved epithelialization, angiogenesis and enhanced collagen deposition by modulating TGF-β3 and MMP - 9 gene expressions favorable for better healing. Thus, this scaffold could be a newer treatment strategy for chronic non-healing wounds.
慢性非愈合性糖尿病创面的典型特征是炎症持续时间长、基质金属蛋白酶升高、缺氧、血管生成减少、氧化应激增加和细菌感染。任何能改善一个或所有因素的药物都能为改善糖尿病创面愈合提供更好的机会。在这项研究中，使用生物相容性交联剂氧化普鲁兰（Col-OxP3-Po）制备了一种含有多酚（虎杖苷）的胶原支架，以治疗糖尿病创面。使用 SEM、FTIR、抗氧化活性、体外和体内伤口愈合试验、基因表达和免疫组织化学研究对支架进行了表征。虎杖苷掺入支架表现出 75%的抗氧化活性、止血和红细胞粘附特性。FTIR 结果证明了虎杖苷在 Col-OxP3-Po 支架中的掺入。它们对 3T3 成纤维细胞也没有毒性，细胞活力为 93%，细胞附着良好。在体内，正常和糖尿病伤口愈合研究表明，Col-OxP3-Po 支架处理组在第 16 天和第 21 天愈合。肉芽组织的组织学和免疫组织化学分析表明，通过调节 TGF-β3 和 MMP-9 基因表达，促进上皮化、血管生成和增强胶原蛋白沉积，有利于更好的愈合。因此，该支架可能是治疗慢性非愈合性创面的一种新的治疗策略。
