Lin Caizhao, Yang Hanjin, Zhao Wenquan, Wang Weibing
Department of Colorectal Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, China.
Department of Pathology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310002, China.
Biochem Biophys Res Commun. 2022 Oct 20;626:8-14. doi: 10.1016/j.bbrc.2022.06.037. Epub 2022 Jul 3.
Colorectal cancer (CRC) is a lethal malignant tumor and 25-30% of CRC patients develop liver metastasis (LM) with a worse prognosis, but the metastasis mechanism is yet elucidated. To identify the potential immune regulatory mechanism of CRC liver metastasis, single-cell sequencing and multiplex immunohistochemistry were applied to identify key cell populations of the tumor microenvironment (TME) in the CRC and LM sites. We found memory CD8 T cells, B cells, and CTSB + macrophages were enriched in the LM site, forming the memory immune hub, which was important for the anti-tumor response against LM. Therefore, our results revealed that memory immune responses were called in the LM sites and probably meditated by CTSB + macrophages.
结直肠癌（CRC）是一种致命的恶性肿瘤，25%-30%的CRC患者会发生肝转移（LM），预后较差，但转移机制尚未阐明。为了确定CRC肝转移的潜在免疫调节机制，采用单细胞测序和多重免疫组化来识别CRC和LM部位肿瘤微环境（TME）的关键细胞群。我们发现记忆性CD8 T细胞、B细胞和CTSB+巨噬细胞在LM部位富集，形成记忆免疫枢纽，这对针对LM的抗肿瘤反应很重要。因此，我们的结果表明，LM部位会引发记忆免疫反应，可能由CTSB+巨噬细胞介导。
