State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, And School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, And School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Environ Res. 2022 Nov;214(Pt 3):114047. doi: 10.1016/j.envres.2022.114047. Epub 2022 Aug 11.
Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene, are a type of organic pollutants that exist widely in the environment. Of the currently known degradation methods, bioremediation is a desirable and feasible option. A novel Diaphorobacter sp. Strain MNS-0 was isolated from saponification wastewater and showed the ability to degrade phenanthrene, fluorene, acenaphthene, anthracene, benzo[a]anthracene, or chrysene using phenanthrene as the sole carbon source. Gas chromatography mass spectroscopy analysis of catabolic intermediates indicates that phenanthrene degradation occurs through the phthalic acid pathway in strain MNS-0. Genome sequencing shows that strain MNS-0 has two plasmids and one chromosome containing a presumptive phenanthrene degradation gene cluster. Strain MNS-0 was able to completely degrade 100 mg/L phenanthrene within 40 h and tolerate up to 10 g/L NaCl at pH 9.0, while maintaining phenanthrene degradation activity. We thus propose that strain MNS-0 is an effective degrader for bioremediation of PAHs pollution, even in relatively harsh alkali environments such as saponification wastewater.
多环芳烃（PAHs），如菲，是一种广泛存在于环境中的有机污染物。在现有的降解方法中，生物修复是一种理想且可行的选择。一种新型鞘氨醇单胞菌（Strain MNS-0）从皂化废水中分离得到，能够以菲作为唯一碳源降解菲、芴、苊、蒽、苯并[a]蒽或䓛。对代谢中间产物的气相色谱质谱分析表明，MNS-0 菌株通过邻苯二甲酸途径降解菲。基因组测序表明，MNS-0 菌株含有两个质粒和一条染色体，其中包含一个推定的菲降解基因簇。MNS-0 菌株能够在 40 小时内完全降解 100mg/L 的菲，并且在 pH9.0 时能够耐受高达 10g/L 的 NaCl，同时保持菲的降解活性。因此，我们提出 MNS-0 菌株是一种有效的多环芳烃污染生物修复剂，即使在皂化废水等相对苛刻的碱性环境中也是如此。
