School of Life Science and Biotechnology, Dalian University of Technology, Liaoning 116023, China.
J Environ Sci (China). 2012;24(12):2141-8. doi: 10.1016/s1001-0742(11)61064-4.
It is important to screen strains that can decompose polycyclic aromatic hydrocarbons (PAHs) completely and rapidly with good adaptability for bioremediation in a local area. A bacterial strain JM2, which uses phenanthrene as its sole carbon source, was isolated from the active sewage sludge from a chemical plant in Jilin, China and identified as Pseudomonas based on 16S rDNA gene sequence analysis. Although the optimal growth conditions were determined to be pH 6.0 and 37 degrees C, JM2 showed a broad pH and temperature profile. At pH 4.5 and 9.3, JM2 could degrade more than 40% of fluorene and phenanthrene (50 mg/L each) within 4 days. In addition, when the temperature was as low as 4 degrees C, JM2 could degrade up to 24% fluorene and 12% phenanthrene. This showed the potential for JM2 to be applied in bioremediation over winter or in cold regions. Moreover, a nutrient augmentation study showed that adding formate into media could promote PAH degradation, while the supplement of salicylate had an inhibitive effect. Furthermore, in a metabolic pathway study, salicylate, phthalic acid, and 9-fluorenone were detected during the degradation of fluorene or phenanthrene. In conclusion, Pseudomonas sp. JM2 is a high performance strain in the degradation of fluorene and phenanthrene under extreme pH and temperature conditions. It might be useful in the bioremediation of PAHs.
筛选能够完全且快速降解多环芳烃 (PAHs)、具有良好适应性的菌株对于在当地进行生物修复非常重要。一株以菲为唯一碳源的细菌 JM2 从中国吉林某化工厂的活性污泥中分离得到，根据 16S rDNA 基因序列分析鉴定为假单胞菌。虽然确定最佳生长条件为 pH6.0 和 37°C，但 JM2 表现出较宽的 pH 和温度范围。在 pH4.5 和 9.3 时，JM2 可在 4 天内降解超过 40%的芴和菲（各 50mg/L）。此外，当温度低至 4°C 时，JM2 可降解高达 24%的芴和 12%的菲。这表明 JM2 有潜力在冬季或寒冷地区进行生物修复。此外，营养增强研究表明，向培养基中添加甲酸盐可促进 PAH 降解，而水杨酸的添加则有抑制作用。此外，在代谢途径研究中，在降解芴或菲时检测到水杨酸、邻苯二甲酸和 9-芴酮。总之，假单胞菌 JM2 是一种在极端 pH 和温度条件下降解芴和菲的高性能菌株。它可能在 PAHs 的生物修复中有用。
