Wang Zan, Hu Haiyang, Zhang Zhan, Xu Yongming, Xu Ping, Tang Hongzhi
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
China Tobacco Henan Industrial Co. Ltd., Zhengzhou 450000, People's Republic of China.
Sci Total Environ. 2023 Jun 25;879:162974. doi: 10.1016/j.scitotenv.2023.162974. Epub 2023 Mar 21.
Polycyclic aromatic hydrocarbons (PAHs) and heterocyclic derivatives are organic pollutants which threaten ecosystems and human beings. In this study, a new strain, Shinella sp. FLN 14, was isolated and characterized. It can utilize fluorene as its sole carbon source and effectively co-metabolize multiple PAHs and heterocyclic derivatives, including phenanthrene, acenaphthene, and fluoranthene. Two possible metabolic pathways are proposed (i.e., salicylic acid pathway and phthalic acid pathway). Whole-genome sequencing revealed that strain FLN14 possesses a chromosome and four plasmids. However, when combined with ensemble genetic information, novel fluorene-degrading functional gene clusters were not located within the genome of FLN 14, except for some new dioxygenases and electron transport chains, which typically initiate the oxidation of aromatic compounds. In wastewater bioremediation, strain FLN14 removed nearly 95 % of PAHs within 5 days and maintained high degrading activity during the 18-day reaction compared to the control. Overall, our study provides a promising candidate to achieve bioremediation of PAHs-contaminated environments.
多环芳烃（PAHs）和杂环衍生物是威胁生态系统和人类的有机污染物。在本研究中，分离并鉴定了一种新菌株，即中华根瘤菌属（Shinella sp.）FLN 14。它能够利用芴作为唯一碳源，并有效地共代谢多种多环芳烃和杂环衍生物，包括菲、苊和荧蒽。提出了两条可能的代谢途径（即水杨酸途径和邻苯二甲酸途径）。全基因组测序表明，菌株FLN14拥有一条染色体和四个质粒。然而，结合整体遗传信息来看，除了一些通常启动芳香族化合物氧化的新双加氧酶和电子传递链外，新型芴降解功能基因簇并不位于FLN 14的基因组内。在废水生物修复中，与对照相比，菌株FLN14在5天内去除了近95%的多环芳烃，并在18天的反应过程中保持了较高的降解活性。总体而言，我们的研究为实现多环芳烃污染环境的生物修复提供了一个有前景的候选菌株。
