From the Department of Clinical Pharmacy (Alshabi) and from the Department of Pharmacology (Shaikh), College of Pharmacy, Najran University, Najran, Kingdom of Saudi Arabia.
Saudi Med J. 2022 Aug;43(8):881-890. doi: 10.15537/smj.2022.43.8.20220258.
To test the antidiabetic potential of extract (GLE) in rats with type 2 diabetes mellitus (T2DM) induced by a high-fat diet (HFD) + streptozotocin (STZ).
The study was carried out in June 2021. powdered leaves were subjected to Soxhlet extraction using ethanol. Male rats were administered a low dose-40 mg/kg STZ by intraperitoneal route following 2 weeks of HFD to induce type-2 diabetic rats (T2DR). Rats were randomized into 5 groups (n=6). Group 1 (normal control; 10 ml/kg normal saline); Group 2 (diabetic control: DC); Group 3 (standard; DR + metformin, 100 mg/kg per oral); Group 4 (DR + GLE 250 mg/kg); Group 5 (DR + GLE 500 mg/kg). The treatment period extended for 2 weeks. Body weight and fasting blood glucose were determined on days 0, 7, and 14. Fasting serum insulin (FSI) levels, fasting blood glucose (FBG), HOMA-IR, antioxidant enzyme level, Insulin tolerance test (ITT), and intraperitoneal glucose tolerance test (IPGTT) tests were estimated.
extract exhibited a marked decrease (<0.001) in fasting blood glucose levels. T2DR receiving a higher dose of GLE showed a greater improvement in metabolic indices (FSI, FBG, Homeostatic Model Assessment of insulin resistance). The ITT and IPGTT results demonstrated that GLE could significantly enhance insulin tolerance, glucose tolerance, and antioxidant enzyme levels in T2DR.
can be an ideal medicinal plant candidate for treating T2DM, and it should be investigated further for its therapeutic potential.
研究高脂饮食（HFD）+链脲佐菌素（STZ）诱导的 2 型糖尿病（T2DM）大鼠中 提取物（GLE）的抗糖尿病潜力。
本研究于 2021 年 6 月进行。将 粉末状叶片用乙醇进行索氏提取。雄性大鼠在 HFD 2 周后通过腹腔途径给予低剂量-40mg/kg STZ 诱导 2 型糖尿病大鼠（T2DR）。大鼠随机分为 5 组（n=6）。第 1 组（正常对照组；10ml/kg 生理盐水）；第 2 组（糖尿病对照组：DC）；第 3 组（标准组；DR+二甲双胍，100mg/kg 口服）；第 4 组（DR+GLE 250mg/kg）；第 5 组（DR+GLE 500mg/kg）。治疗期延长至 2 周。分别在第 0、7 和 14 天测定体重和空腹血糖。测定空腹血清胰岛素（FSI）水平、空腹血糖（FBG）、HOMA-IR、抗氧化酶水平、胰岛素耐量试验（ITT）和腹腔葡萄糖耐量试验（IPGTT）。
提取物可显著降低（<0.001）空腹血糖水平。接受较高剂量 GLE 的 T2DR 显示出对代谢指数（FSI、FBG、稳态模型评估的胰岛素抵抗）的更大改善。ITT 和 IPGTT 结果表明，GLE 可显著增强 T2DR 的胰岛素耐量、葡萄糖耐量和抗氧化酶水平。
可能是治疗 T2DM 的理想药用植物候选物，应进一步研究其治疗潜力。
