Key Laboratory of Agricultural Water Resources, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences.
University of Chinese Academy of Sciences.
Microbes Environ. 2022;37(3). doi: 10.1264/jsme2.ME22035.
Ammonia-oxidizing archaea (AOA), key players in agricultural upland soil nitrification, convert soil ammonium to nitrite. The microbial oxidation of ammonia to nitrite is an important part of the global biogeochemical nitrogen cycle. In the present study, we recovered six novel AOA metagenome-assembled genomes (MAGs) containing genes for carbon (C) fixation and nitrogen (N) metabolism by using a deep shotgun metagenomic sequencing strategy. We also found that these AOA MAGs possessed cobalamin synthesis genes, suggesting that AOA are vitamin suppliers in agricultural upland soil. Collectively, the present results deepen our understanding of the metabolic potential and phylogeny of AOA in agroecosystems.
氨氧化古菌（AOA）是农业旱地土壤硝化作用的关键参与者，它们将土壤中的铵转化为亚硝酸盐。氨微生物氧化为亚硝酸盐是全球生物地球化学氮循环的重要组成部分。在本研究中，我们通过深度 shotgun 宏基因组测序策略，从土壤中回收了六个包含碳（C）固定和氮（N）代谢基因的新型氨氧化古菌宏基因组组装基因组（MAG）。我们还发现这些氨氧化古菌 MAG 含有钴胺素合成基因，表明氨氧化古菌是农业旱地土壤中的维生素供应者。总之，本研究结果加深了我们对农业生态系统中氨氧化古菌代谢潜力和系统发育的理解。
