Super-cutting-edge Grand and Advanced Research (Sugar) Program, Institute for Extra-cutting-edge Science and Technology Avant-garde Research (X-star), Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
Laboratory of Marine Environmental Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University.
Microbes Environ. 2023;38(4). doi: 10.1264/jsme2.ME23052.
Infrared spectroscopy is used for the chemical characterization of prokaryotes. However, its application has been limited to cell aggregates and lipid extracts because of the relatively low spatial resolution of diffraction. We herein report optical photothermal infrared (O-PTIR) spectroscopy of prokaryotes for a domain-level diagnosis at the single-cell level. The technique provided infrared spectra of individual bacterial as well as archaeal cells, and the resulting aliphatic CH/CH intensity ratios showed domain-specific signatures, which may reflect distinctive cellular lipid compositions; however, there was interference by other cellular components. These results suggest the potential of O-PTIR for a domain-level diagnosis of single prokaryotic cells in natural environments.
红外光谱用于原核生物的化学特征分析。然而，由于其衍射的空间分辨率相对较低，该技术的应用一直局限于细胞聚集体和脂类提取物。在此，我们报告了用于单细胞水平的域级诊断的光学光热红外（O-PTIR）光谱技术。该技术提供了单个细菌和古细菌细胞的红外光谱，所得的脂肪族 CH/CH 强度比表现出具有特征的域特异性，这可能反映了独特的细胞脂质组成；然而，其他细胞成分也会产生干扰。这些结果表明 O-PTIR 有可能用于对自然环境中单一个原核细胞进行域级诊断。
