Lee Yun-Ju, Pak Hayeon, Hwang Chung-Ju, Choi Yoon Jeong, Lee Jae-Hoon, Lee Ji Hyun, Cha Jung-Yul
Department of Orthodontics, Yonsei University College of Dentistry, Seodaemoon-gu, Seoul, South Korea.
Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University, Dongdaemoon-gu, Seoul, South Korea.
Am J Orthod Dentofacial Orthop. 2022 Nov;162(5):668-679.e5. doi: 10.1016/j.ajodo.2021.06.022. Epub 2022 Aug 11.
External apical root resorption (EARR) is one of the most common unfavorable consequences of orthodontic treatment and causes loss of tooth structure. The present study aimed to investigate the genetics of EARR using next-generation sequencing comprehensively.
Targeted next-generation sequencing was performed for comprehensive genetic analysis of 118 Korean orthodontic patients. The patients were divided into 2 groups on the basis of their EARR value. The association of clinical and genetic parameters with EARR was assessed using the χ test or t test for matched pairs, followed by Bonferroni correction and linear regression analysis. In addition, haplotype analysis and in silico prediction were conducted to evaluate functional effects.
No statistically significant difference was observed between clinical and treatment-related parameters and EARR. The single nucleotide polymorphisms SPP1 rs9138 (P = 0.001) and SFRP2 rs3810765 (P = 0.04) showed only nominal significance between EARR groups. However, these 2 SNPs were not significant after Bonferroni correction for multiple testing (cutoff P = 0.05/142 = 3.52 × 10). Variations in SPP1 rs9138 and SFRP2 rs3810765 may be related to EARR during orthodontic treatment. In summary, not only genes related to inflammatory reactions but also those related to Wnt signaling to affect the degree of EARR during orthodontic teeth movement.
根尖外吸收（EARR）是正畸治疗最常见的不良后果之一，会导致牙体结构丧失。本研究旨在通过下一代测序全面探究EARR的遗传学机制。
对118名韩国正畸患者进行靶向性下一代测序，以进行全面的基因分析。根据EARR值将患者分为两组。采用χ检验或配对t检验评估临床和基因参数与EARR的相关性，随后进行Bonferroni校正和线性回归分析。此外，进行单倍型分析和计算机模拟预测以评估功能效应。
临床和治疗相关参数与EARR之间未观察到统计学上的显著差异。单核苷酸多态性SPP1 rs9138（P = 0.001）和SFRP2 rs3810765（P = 0.04）在EARR组之间仅显示出名义上的显著性。然而，经过多重检验的Bonferroni校正（临界P = 0.05/142 = 3.52×10）后，这两个单核苷酸多态性并不显著。SPP1 rs9138和SFRP2 rs3810765的变异可能与正畸治疗期间的EARR有关。总之，在正畸牙齿移动过程中，不仅与炎症反应相关的基因，而且与Wnt信号通路相关的基因都会影响EARR的程度。
