Dentist's Office ORTO-PUNKT, Mościckiego St. 72/1, 33-100 Tarnów, Poland.
Department of Integrated Pediatric Dentistry, Chair of Integrated Dentistry, Medical University of Lublin, 20-059 Lublin, Poland.
Int J Mol Sci. 2021 Jan 14;22(2):777. doi: 10.3390/ijms22020777.
External apical root resorption (EARR) is one of the most serious complications associated with orthodontic treatment. The aim of the study was to analyze the relationships between selected single nucleotide polymorphisms (SNPs) in Interleukin 1 receptor antagonist (), purinoreceptor P2X7 () and EARR in patients after orthodontic treatment. The study comprised 101 patients who underwent a complex orthodontic treatment with a combination of fixed appliances. Roots were measured based on orthopantomograms and lateral cephalometric radiographs taken before and at the end of the treatment using diagnostic software. Proportional measurements of selected teeth were made using the modified Linge and Linge methods. Based on the presence or absence of EARR, patients were divided into two groups: control group, 61 patients without EARR (with 0.90 ≤ rRCR ≤ 1.00), and EARR group, 40 patients with EARR (rRCR < 0.90). Root resorption in selected groups was also evaluated with the scores of Malmgren and Levander. SNP analysis was performed using the real-time polymerase chain reaction (PCR) method. The analysis indicated that a specific haplotype of (rs208294) and (rs419598) modified the risk of EARR development ( < 0.05), with a Bonferroni correction. The analysis of the and gene polymorphisms showed that the presence of SNPs of these genes may predispose individuals to EARR. These findings indicate that EARR is a complex condition influenced not only by environmental factors and needs further study on the genetic risk factors.
外部根尖吸收（EARR）是正畸治疗相关的最严重并发症之一。本研究旨在分析白细胞介素 1 受体拮抗剂（）、嘌呤受体 P2X7（）中选定的单核苷酸多态性（SNPs）与正畸治疗后 EARR 之间的关系。该研究纳入了 101 名接受综合正畸治疗的患者，治疗方案包括固定矫治器。使用诊断软件，根据治疗前后的全景片和侧位头颅侧位片测量牙根。使用改良的林格和林格方法对选定牙齿进行比例测量。根据 EARR 的有无，将患者分为两组：对照组 61 例，EARR 为 0.90≤rRCR≤1.00；EARR 组 40 例，EARR 为 rRCR<0.90。还使用 Malmgren 和 Levander 评分评估了选定组的牙根吸收情况。使用实时聚合酶链反应（PCR）方法进行 SNP 分析。分析表明，（rs208294）和（rs419598）的特定单倍型改变了 EARR 发展的风险（<0.05），经 Bonferroni 校正。对和基因多态性的分析表明，这些基因的 SNP 存在可能使个体易患 EARR。这些发现表明 EARR 是一种复杂的疾病，不仅受环境因素的影响，还需要进一步研究遗传风险因素。
