Clinical Biochemical Laboratory, Hospital of Bolzano, Bolzano, Italy.
Northern Ireland Blood Transfusion Service, Belfast, UK.
Biochem Med (Zagreb). 2022 Oct 1;32(3):030802. doi: 10.11613/BM.2022.030802. Epub 2022 Aug 5.
Screening and measurement of monoclonal (M) proteins are commonly performed using capillary zone electrophoresis (CZE). The identification of M-protein or monoclonal component (CM) is an essential requirement for diagnosis and monitoring of monoclonal gammopathies. The detection of CM has been largely improved by CZE. Capillary electrophoresis estimates CM more accurately, because absence of variation due to different dye binding affinities of proteins as instead seen with agarose gel electrophoresis. However, interferences can be present in CZE. This occurs because all substances absorbing at 200 nm can be identified. Recognition and handling of specimens exhibiting such interferences is essential to ensure accurate diagnostic and patient safety. We herein report on an unusual case of serum protein electrophoresis, to highlight that laboratory staff must be aware of and familiarise with the information provided by laboratory instruments. For example, in the case of serum indices, about specimen quality.
使用毛细管区带电泳（CZE）通常可进行单克隆（M）蛋白的筛选和测量。M 蛋白或单克隆成分（CM）的鉴定是诊断和监测单克隆丙种球蛋白病的必要条件。CZE 大大提高了 CM 的检测能力。毛细管电泳更准确地估计 CM，因为不存在由于蛋白质与染料结合亲和力的不同而导致的差异，而琼脂糖凝胶电泳则存在这种差异。然而，CZE 中可能存在干扰。这是因为可以识别所有在 200nm 处吸收的物质。识别和处理表现出这种干扰的标本对于确保准确的诊断和患者安全至关重要。我们在此报告了一个不寻常的血清蛋白电泳病例，以强调实验室人员必须了解和熟悉实验室仪器提供的信息。例如，在血清指标方面，关于标本质量。
