Lian Yifei, Fu Hongran, Xu Xiaoyi, Ju Chuanyu
Mudanjiang Medical University, No. 3, Tongxiang Street, Aimin District, Mudanjiang City, Heilongjiang Province, China.
Hongqi Affiliated Hospital, Mudanjiang Medical University, No. 5, Tongxiang Street, Aimin District, Mudanjiang City, Heilongjiang Province, China.
Evid Based Complement Alternat Med. 2022 Aug 4;2022:9371497. doi: 10.1155/2022/9371497. eCollection 2022.
To explore the validity and accuracy of electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA) in the serological detection of the hepatitis B virus.
From 6 February 2019 to 1 March 2020, 96 patients diagnosed with hepatitis B virus infection in our hospital were recruited and assigned at a ratio of 1 : 1 to experimental groups A (GA) and B (GB), with 48 cases in each group, and the five major serological indicators of hepatitis B were tested and analyzed using ECLIA and ELISA. In addition, 50 suspected patients were selected for two tests, respectively, to compare the accuracy of the two test methods.
ECLIA was associated with significantly higher expression levels and higher detection rates of HBeAg, HBeAb, HBsAg, and HBsAb versus ELISA ( < 0.05), and the difference in the expression and detection rates of HBcAb levels between the two groups did not come up to the statistical standard ( > 0.05). ECLIA yielded significantly higher sensitivity and specificity than ELISA ( < 0.05), while the two methods showed comparable detection accuracy ( > 0.05).
Despite the inconsistent results of the latest studies on the serological detection of hepatitis B by the two techniques, ECLIA is consistently superior to ELISA and provides better diagnostic benefits and merits promotion.
探讨电化学发光免疫分析法（ECLIA）和酶联免疫吸附测定法（ELISA）在乙型肝炎病毒血清学检测中的有效性和准确性。
选取2019年2月6日至2020年3月1日在我院确诊为乙型肝炎病毒感染的96例患者，按1∶1比例分为实验组A（GA）和实验组B（GB），每组48例，采用ECLIA和ELISA检测并分析乙型肝炎五项主要血清学指标。另外，选取50例疑似患者分别进行两种检测，比较两种检测方法的准确性。
与ELISA相比，ECLIA检测HBeAg、HBeAb、HBsAg和HBsAb的表达水平及检出率显著更高（<0.05），两组间HBcAb水平的表达及检出率差异未达到统计学标准（>0.05）。ECLIA的敏感性和特异性显著高于ELISA（<0.05），而两种方法的检测准确性相当（>0.05）。
尽管最新研究表明两种技术在乙型肝炎血清学检测中的结果不一致，但ECLIA始终优于ELISA，具有更好的诊断效益，值得推广。
