种间保守的单核细胞生物学：来自牛的功能见解。

Monocyte biology conserved across species: Functional insights from cattle.

机构信息

Institute of Virology and Immunology, Bern, Switzerland.

Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

出版信息

Front Immunol. 2022 Jul 29;13:889175. doi: 10.3389/fimmu.2022.889175. eCollection 2022.

Similar to human monocytes, bovine monocytes can be split into CD14CD16 classical, CD14CD16 intermediate and CD14CD16 nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM.

类似于人类单核细胞，牛单核细胞可分为 CD14⁺⁺CD16⁻经典型、CD14⁺⁺CD16⁺中间型和 CD14⁺⁺CD16⁻非经典型单核细胞（cM、intM 和 ncM，分别）。在此，我们对其稳态批量和单细胞转录组进行了深入分析，突出了其明显的功能特化和转录组相关性。批量基因转录表明 cM 具有促炎和抗菌作用，而 ncM 和 intM 似乎专门用于调节/抗炎功能和组织修复，以及抗病毒反应和 T 细胞免疫调节。值得注意的是，intM 表现出与抗原呈递相关的几个基因的高表达。ncM 的抗炎和抗病毒功能还分别得到了主导氧化磷酸化和对 TLR7/8 配体选择性强反应的支持。此外，单细胞 RNA-seq 揭示了 cM 内以前未被认识的异质性，并提出 intM 是 cM 和 ncM 之间的短暂分化中间型。