Institute of Virology and Immunology, Bern, Switzerland.
Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Front Immunol. 2022 Jul 29;13:889175. doi: 10.3389/fimmu.2022.889175. eCollection 2022.
Similar to human monocytes, bovine monocytes can be split into CD14CD16 classical, CD14CD16 intermediate and CD14CD16 nonclassical monocytes (cM, intM, and ncM, respectively). Here, we present an in-depth analysis of their steady-state bulk- and single-cell transcriptomes, highlighting both pronounced functional specializations and transcriptomic relatedness. Bulk gene transcription indicates pro-inflammatory and antibacterial roles of cM, while ncM and intM appear to be specialized in regulatory/anti-inflammatory functions and tissue repair, as well as antiviral responses and T-cell immunomodulation. Notably, intM stood out by high expression of several genes associated with antigen presentation. Anti-inflammatory and antiviral functions of ncM are further supported by dominant oxidative phosphorylation and selective strong responses to TLR7/8 ligands, respectively. Moreover, single-cell RNA-seq revealed previously unappreciated heterogeneity within cM and proposes intM as a transient differentiation intermediate between cM and ncM.
类似于人类单核细胞，牛单核细胞可分为 CD14++CD16−经典型、CD14++CD16+中间型和 CD14++CD16−非经典型单核细胞（cM、intM 和 ncM，分别）。在此，我们对其稳态批量和单细胞转录组进行了深入分析，突出了其明显的功能特化和转录组相关性。批量基因转录表明 cM 具有促炎和抗菌作用，而 ncM 和 intM 似乎专门用于调节/抗炎功能和组织修复，以及抗病毒反应和 T 细胞免疫调节。值得注意的是，intM 表现出与抗原呈递相关的几个基因的高表达。ncM 的抗炎和抗病毒功能还分别得到了主导氧化磷酸化和对 TLR7/8 配体选择性强反应的支持。此外，单细胞 RNA-seq 揭示了 cM 内以前未被认识的异质性，并提出 intM 是 cM 和 ncM 之间的短暂分化中间型。
