College of Fishery, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang, China.
Institute of Forensic Medicine and Laboratory Medicine, Jining Medical University, Jining, China.
Front Immunol. 2022 Jul 28;13:944388. doi: 10.3389/fimmu.2022.944388. eCollection 2022.
Serotonin (5-hydroxytryptamine) is a well-known neurotransmitter affecting emotion, behavior, and cognition. Additionally, numerous immunomodulatory functions of serotonin have been discovered in mammals. However, the regulatory role of the serotonin system in fish immunity remains unclear. In this study, various serotonergic markers in Nile tilapia () were identified and characterized. The involvement of the serotonin system during bacterial infection was investigated. Moreover, the expression characteristics and specific functions of serotonergic markers within Nile tilapia immune cells were also assessed. Overall, 22 evolutionarily conserved serotonergic marker genes in Nile tilapia were cloned and characterized. Transcriptional levels of these molecules were most abundant in the brain, and their transcripts were induced during infection. Nevertheless, few serotonergic markers exist on Nile tilapia immune cells, and no distinct immunomodulation effect was observed during an immune response. The present study lays a theoretical foundation for further investigation of the immunological mechanisms in fish as well as the evolution of the serotonin system in animals.
血清素（5-羟色胺）是一种众所周知的神经递质，影响情绪、行为和认知。此外，在哺乳动物中已经发现了血清素的许多免疫调节功能。然而，血清素系统在鱼类免疫中的调节作用尚不清楚。在这项研究中，鉴定和表征了尼罗罗非鱼中的各种血清素标记物。研究了血清素系统在细菌感染过程中的作用。此外，还评估了尼罗罗非鱼免疫细胞中血清素标记物的表达特征和特定功能。总的来说，克隆并表征了尼罗罗非鱼中 22 个进化上保守的血清素标记基因。这些分子的转录水平在大脑中最为丰富，并且在 感染过程中被诱导。然而，尼罗罗非鱼免疫细胞中存在的血清素标记物很少，在免疫反应过程中也没有观察到明显的免疫调节作用。本研究为进一步研究鱼类的免疫学机制以及动物血清素系统的进化奠定了理论基础。
