Gottlieb Susan, Rand Jacquie S, Ishioka Katsumi, Dias Daniel A, Boughton Berin A, Roessner Ute, Ramadan Ziad, Anderson Stephen T
The Cat Clinic, Brisbane, QLD, Australia.
School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia.
Front Vet Sci. 2022 Jul 29;9:905929. doi: 10.3389/fvets.2022.905929. eCollection 2022.
Firstly, to compare differences in insulin, adiponectin, leptin, and measures of insulin sensitivity between diabetic cats in remission and healthy control cats, and determine whether these are predictors of diabetic relapse. Secondly, to determine if these hormones are associated with serum metabolites known to differ between groups. Thirdly, if any of the hormonal or identified metabolites are associated with measures of insulin sensitivity.
Twenty cats in diabetic remission for a median of 101 days, and 21 healthy matched control cats.
A casual blood glucose measured on admission to the clinic. Following a 24 h fast, a fasted blood glucose was measured, and blood sample taken for hormone (i.e., insulin, leptin, and adiponectin) and untargeted metabolomic (GC-MS and LC-MS) analysis. A simplified IVGGT (1 g glucose/kg) was performed 3 h later. Cats were monitored for diabetes relapse for at least 9 months (270 days).
Cats in diabetic remission had significantly higher serum glucose and insulin concentrations, and decreased insulin sensitivity as indicated by an increase in HOMA and decrease in QUICKI and Bennett indices. Leptin was significantly increased, but there was no difference in adiponectin (or body condition score). Several significant correlations were found between insulin sensitivity indices, leptin, and serum metabolites identified as significantly different between remission and control cats. No metabolites were significantly correlated with adiponectin. No predictors of relapse were identified in this study.
Insulin resistance, an underlying factor in diabetic cats, persists in diabetic remission. Cats in remission should be managed to avoid further exacerbating insulin resistance.
首先，比较糖尿病缓解期猫与健康对照猫在胰岛素、脂联素、瘦素及胰岛素敏感性指标方面的差异，并确定这些指标是否为糖尿病复发的预测因素。其次，确定这些激素是否与已知在两组间存在差异的血清代谢物相关。第三，确定是否有任何激素或已鉴定的代谢物与胰岛素敏感性指标相关。
20只糖尿病缓解期猫，缓解期中位数为101天，以及21只健康匹配对照猫。
入院时测定随机血糖。禁食24小时后，测定空腹血糖，并采集血样用于激素（即胰岛素、瘦素和脂联素）及非靶向代谢组学（气相色谱 - 质谱联用仪和液相色谱 - 质谱联用仪）分析。3小时后进行简化的静脉葡萄糖耐量试验（1克葡萄糖/千克）。对猫进行至少9个月（270天）的糖尿病复发监测。
糖尿病缓解期猫的血清葡萄糖和胰岛素浓度显著更高，且胰岛素敏感性降低，表现为稳态模型评估（HOMA）增加、定量胰岛素敏感性检查指数（QUICKI）和贝内特指数降低。瘦素显著升高，但脂联素（或身体状况评分）无差异。在胰岛素敏感性指标、瘦素与缓解期猫和对照猫之间差异显著的血清代谢物之间发现了几个显著相关性。没有代谢物与脂联素显著相关。本研究未发现复发的预测因素。
胰岛素抵抗是糖尿病猫的一个潜在因素，在糖尿病缓解期持续存在。应对缓解期的猫进行管理，以避免进一步加重胰岛素抵抗。
