Panneerselvam Karthika, Karthik Raghavendhar K, Ramadoss Ramya, Kumar A Ramesh, Rajkumar K
Oral Pathology and Microbiology, Karpaga Vinayaga Institute of Dental Sciences, Kanchipuram, Tamil Nadu, India.
Oral Pathology and Microbiology, SRM Dental College and Hospital, Chennai, Tamil Nadu, India.
J Oral Maxillofac Pathol. 2022 Apr-Jun;26(2):285. doi: 10.4103/jomfp.JOMFP_135_20. Epub 2022 Jun 28.
Papanicolaou (Pap) staining technique is a conventional technique used in cytology but it is time consuming.
The aim of the study was to determine the efficacy of Rapid Economical Acetic acid Papanicolaou stain (REAP) over conventional staining technique in studying normal oral mucosa.
Eighty patients were selected and were grouped based on their habits. Two smears were obtained from each patient and were subjected to both the staining techniques. A total of 160 slides were studied for features such as cellular outline, nuclear outline, nuclear details, cellular differentiation, micronuclei and cellular transparency.
The conventional staining procedure showed 79 cases of optimal cellular staining, 78 cases of optimal nuclear staining, 35 cases of optimal nuclear details, 57 cases of optimal differentiation and 27 cases of optimal transparency and 33 cases of optimal micronuclei. REAP staining showed 75 cases of optimal cellular staining, 64 cases of optimal nuclear staining, 20 cases of optimal nuclear details, 36 cases of optimal differentiation and 28 cases of optimal transparency and 25 cases of optimal micronuclei.
REAP staining effectively reduces the time and the cost factor, but the cytological details are well observed under conventional staining technique in normal patients.
巴氏染色技术是细胞学中常用的传统技术，但耗时较长。
本研究旨在确定快速经济醋酸巴氏染色（REAP）在研究正常口腔黏膜方面相对于传统染色技术的效果。
选取80例患者，根据其习惯进行分组。从每位患者获取两张涂片，并对两种染色技术均进行处理。共对160张玻片进行细胞轮廓、核轮廓、核细节、细胞分化、微核及细胞透明度等特征的研究。
传统染色程序显示79例细胞染色最佳、78例核染色最佳、35例核细节最佳、57例分化最佳、27例透明度最佳以及33例微核最佳。REAP染色显示75例细胞染色最佳、64例核染色最佳、20例核细节最佳、36例分化最佳、28例透明度最佳以及25例微核最佳。
REAP染色有效减少了时间和成本因素，但在正常患者中，传统染色技术下的细胞学细节观察效果更佳。
