Varki N M, Roome L, Sparkes R S, Miller J E
Int J Cancer. 1987 Jul 15;40(1):46-52. doi: 10.1002/ijc.2910400109.
A human lung carcinoma cell line, UCP3, was carried as subcutaneous xenotransplants in athymic mice. Autopsies of these animals showed rare foci of microscopically visible metastases to any of the other organs. A metastatic variant, 522, was established serendipitously in vitro as a continuous cell line by blind isolation of the pulmonary metastatic foci, at the time of autopsy, from the lungs of the animals that carried subcutaneous xenotransplants of the parental cell line. The parental UCP3 and the metastatic variant 522 were examined by karyotypic and isoenzyme analysis and shown to be human and related. The metastatic variant, 522, metastasizes spontaneously from subcutaneous sites (like the parental UCP3). However, it forms larger subcutaneous xenotransplants and forms more metastatic foci in the lungs of the animals than does the parental cell line. Comparisons of the cell surface glycolipids show many similarities and a few differences. This model system may now be used for further investigations into the processes of metastasis of this human neoplasm.
将人肺癌细胞系UCP3作为皮下异种移植物接种于无胸腺小鼠体内。对这些动物进行尸检发现，显微镜下可见的转移灶罕见于其他任何器官。在尸检时，通过从携带亲代细胞系皮下异种移植物的动物肺部盲目分离肺转移灶，意外地在体外建立了一种转移性变体522，作为连续细胞系。通过核型和同工酶分析对亲代UCP3和转移性变体522进行检测，结果表明它们均为人源且具有相关性。转移性变体522可从皮下部位自发转移（与亲代UCP3一样）。然而，与亲代细胞系相比，它能形成更大的皮下异种移植物，且在动物肺部形成更多的转移灶。对细胞表面糖脂的比较显示出许多相似之处和一些差异。该模型系统现在可用于对这种人类肿瘤转移过程的进一步研究。
