Corti C, Pratesi G, DeCesare M, Pellegrini R, Giardini R, Supino R, Zunino F
Division of Experimental Oncology B, Istituto Nazionale Tumori, Milan, Italy.
J Cancer Res Clin Oncol. 1996;122(3):154-60. doi: 10.1007/BF01366955.
The cell line NIH-H460, derived from a human large-cell carcinoma of the lung, because metastatic in the lung of athymic mice after S.C. injection. Using serial S.C. passages of the lung metastasis, a cell line was selected, H460M, which was characterized by an increased metastatic potential compared to the parental cells after s.c. (Spontaneous metastases) or i.v. injection (experimental metastases). For the high frequency of lung metastases in the mouse and the short time required to develop metastatic foci, the H460M cells transplanted in nude mice represent a unique preclinical model for biological and therapeutic studies. The paper describes the growth and some biological properties (invasion and migration capability, membrane profile) of this experimental in vivo model.
细胞系NIH-H460源自人肺大细胞癌，皮下注射后可在无胸腺小鼠肺内转移。通过对肺转移灶进行连续皮下传代，筛选出一种细胞系H460M，与亲代细胞相比，皮下注射（自发转移）或静脉注射（实验性转移）后，该细胞系具有更高的转移潜能。由于小鼠肺转移频率高且形成转移灶所需时间短，移植到裸鼠体内的H460M细胞代表了一种用于生物学和治疗研究的独特临床前模型。本文描述了该实验性体内模型的生长情况及一些生物学特性（侵袭和迁移能力、膜特征）。
