CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, People's Republic of China.
College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, People's Republic of China.
Anal Chem. 2022 Aug 23;94(33):11564-11572. doi: 10.1021/acs.analchem.2c01832. Epub 2022 Aug 14.
Ribose plays an important role in the process of life. Excessive ribose in the human cerebrospinal fluid or urine can be used as an early diagnostic marker of leukoencephalopathy. Fluorinated phenylboronic acid combined with F NMR spectroscopy was a powerful method for molecular recognition. However, phenylboronic acid-based sensors for selective detection of ribose are rarely reported in the literature. In this study, the rapid and highly selective recognition of ribose was studied by F NMR and 2-fluorophenylboric acid. It was found that 2-fluoro-phenylboric acid was an appropriate F NMR-based sensor molecule for the determination of ribose under physiological conditions with high selectivity and robust anti-interference ability. When 2-fluorophenylboric acid was used for the detection of ribose in human urine without any sample pretreatment, a limit of detection of 78 μM was obtained at room temperature under given F NMR experimental conditions (400 MHz, 512 scans, ca. 12 min), which can well meet the needs of practical application.
核糖在生命过程中扮演着重要的角色。人体脑脊液或尿液中过量的核糖可用作脑白质病的早期诊断标志物。氟化苯硼酸与 F NMR 光谱相结合是一种强大的分子识别方法。然而，文献中很少有报道基于苯硼酸的传感器可用于选择性检测核糖。在这项研究中，通过 F NMR 和 2-氟苯硼酸研究了核糖的快速和高选择性识别。结果发现，在生理条件下，2-氟苯硼酸是一种合适的基于 F NMR 的传感器分子，对核糖具有高选择性和强大的抗干扰能力。当 2-氟苯硼酸用于检测未经任何样品预处理的人尿中的核糖时，在给定的 F NMR 实验条件（400 MHz，512 次扫描，约 12 分钟）下，在室温下获得了 78 μM 的检测限，这可以很好地满足实际应用的需求。
