Shekatkar Madhura, Kheur Supriya, Sanap Avinash, Undale Vaishali, Kharat Avinash, Bhalchim Vrushali, Dubewar Arati, Bhonde Ramesh
Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India.
Regenerative Medicine Laboratory, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pimpri, Pune, India.
Med Oncol. 2022 Aug 16;39(11):162. doi: 10.1007/s12032-022-01760-6.
Epidemiological data have proved the association of consumption of areca nut with the causation of oral submucous fibrosis (OSF). OSF is a chronic inflammatory disease with the potential for malignant transformation from 7 to 13%. The establishment of animal models makes it easier for researchers to focus on the therapeutic options to combat this disease further. We developed and compared two areca nut extract (ANE) administration methods in Swiss albino mice to establish OSF. This study compared an invasive intrabuccal injection technique with a non-invasive intraoral droplet administration. The duration of induction was around 12 weeks. Histopathology (H&E, Masson's trichrome staining) and gene expression analysis (COL-I, COL-II, and α-SMA) were performed using RT-PCR to confirm the OSF in animals. Our study showed that ANE administration through the intraoral droplet method exhibited significantly higher fibrosis than the intrabuccal injections, as evidenced by the H&E and Masson's trichrome staining. Furthermore, intraoral administration of ANE significantly upregulated the mRNA expression of COL-I, COL-II, and α-SMA, as revealed by the RT-PCR analysis. The non-invasive droplet method could simulate the absorption of areca nut seen in humans through daily dosing. This study establishes the intraoral droplet method as an efficient and non-invasive method to administer the ANE to develop OSF. These findings will aid in the efficient development of OSF animal models for interventional studies, including screening novel drugs in the reversal of the OSF.
流行病学数据已证实食用槟榔与口腔黏膜下纤维化（OSF）的病因有关。OSF是一种慢性炎症性疾病，有7%至13%的恶变可能性。动物模型的建立使研究人员更容易专注于进一步对抗这种疾病的治疗选择。我们在瑞士白化小鼠中开发并比较了两种槟榔提取物（ANE）给药方法以建立OSF。本研究比较了侵入性颊内注射技术与非侵入性口腔内滴注给药。诱导持续时间约为12周。使用RT-PCR进行组织病理学（苏木精-伊红染色、Masson三色染色）和基因表达分析（I型胶原蛋白、II型胶原蛋白和α-平滑肌肌动蛋白）以确认动物中的OSF。我们的研究表明，通过口腔内滴注法给予ANE比颊内注射表现出明显更高的纤维化，苏木精-伊红染色和Masson三色染色证明了这一点。此外，RT-PCR分析显示，ANE的口腔内给药显著上调了I型胶原蛋白、II型胶原蛋白和α-平滑肌肌动蛋白的mRNA表达。非侵入性滴注法可以模拟人类通过每日给药对槟榔的吸收。本研究确立了口腔内滴注法作为一种有效且非侵入性的给予ANE以诱导OSF的方法。这些发现将有助于高效开发用于干预研究的OSF动物模型，包括筛选逆转OSF的新药。
