Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005487.
Two aerobic, Gram-stain-negative, motile, mesophilic, rod-shaped and catalase-positive bacterial strains designated AF9R3 and GN2-R2 were isolated from flowers collected in the Republic of Korea. Strain AF9R3 grew at 4-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v), and strain GN2-R2 grew at 10-33 °C, pH 4.0-9.0 and with 0-1 % NaCl (w/v). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strains AF9R3 and GN2-R2 belonged to the genera and , respectively, showing high sequence similarity to CY42W (99.4 %) and 6 NM-7 (98.0 %), respectively. Both strains contained summed feature 3 (C 7 and/or C 6) and C as the major fatty acids, and ubiquinone Q-8 as the predominant quinone. Strain AF9R3 had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and strain GN2-R2 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the major polar lipids. Orthologous average nucleotide identity and digital DNA-DNA hybridization values of strain AF9R3 to its closest relative CY42W were 92.6 and 56.5 %, and those of strain GN2-R2 to its closest relative 6 NM-7 were 81.4 and 24.8 %. Based on genotypic and phenotypic data, strains AF9R3 and GN2-R2 are considered to represent novel species of the genus and , respectively, for which the names sp. nov. (type strain AF9R3=KACC 21258=NBRC 114510) and sp. nov. (type strain GN2-R2=KACC 21261=NBRC 114511) have been proposed.
两株好氧、革兰氏阴性、能运动、中温、杆状、接触酶阳性的细菌菌株 AF9R3 和 GN2-R2 从韩国采集的花朵中分离得到。菌株 AF9R3 在 4-33°C、pH 值 4.0-9.0 和 0-1%NaCl（w/v）下生长，菌株 GN2-R2 在 10-33°C、pH 值 4.0-9.0 和 0-1%NaCl（w/v）下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 AF9R3 和 GN2-R2 分别属于 和 属，与 CY42W（99.4%）和 6 NM-7（98.0%）的序列相似性较高。两株菌均含有特征脂肪酸总和 3（C 7 和/或 C 6）和 C，主要醌类为泛醌 Q-8。菌株 AF9R3 含有二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺，而菌株 GN2-R2 含有二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和一种未鉴定的磷脂作为主要极性脂。菌株 AF9R3 与其最接近的种 CY42W 的同源平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 92.6%和 56.5%，菌株 GN2-R2 与其最接近的种 6 NM-7 的同源平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 81.4%和 24.8%。基于基因型和表型数据，菌株 AF9R3 和 GN2-R2 分别被认为是 属和 属的新种，分别命名为 sp. nov.（模式菌株 AF9R3=KACC 21258=NBRC 114510）和 sp. nov.（模式菌株 GN2-R2=KACC 21261=NBRC 114511）。
