Lu Huibin, Deng Tongchu, Liu Feifei, Wang Yonghong, Yang Xunan, Xu Meiying
Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070, PR China.
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2020 Aug;70(8):4822-4830. doi: 10.1099/ijsem.0.004355.
Five Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT50W, FT80W, FT92W, FT94W and FT135W) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT50W, FT94W and FT135W take strain Sac-22, and strains FT80W and FT92W take strain DCY83 as their closest neighbour in the phylogenetic trees, respectively. The G+C contents of strains FT50W, FT80W, FT92W, FT94W and FT135W were 63.3, 62.4, 62.8, 63.8 and 60.8 %, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strains FT50W, FT80W, FT94W and FT135W clustered together with species of the genus , but strains FT92W and KCTC 42409 were located in the clades of the genus . The calculated pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among strains FT50W, FT80W, FT92W, FT94W, FT135W and related strains were in the ranges of 75.6-87.8% and 20.3-33.8% except that the values between strains FT50W and FT94W were 98.7 and 89.2%, respectively. The respiratory quinone of these five strains was Q-8. The major fatty acids were C 7, C, C ω7 and C. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Considering the distinct phylogenetic relationships of with species of the genus in the phylogenomic tree, it was reasonable to transfer to the genus as comb. nov. Combining the results of phylogenomic analysis, ANI and dDDH data, and a range of physiological and biochemical characteristics together, strains FT50W and FT94W should belong to the same species and be assigned to genus with strains FT80W and FT135W together, and strain FT92W should be assigned to the genus , for which the names sp. nov. (type strain FT50W=GDMCC 1.1674=KACC 21466), sp. nov. (FT80W=GDMCC 1.1678=KACC 21470), sp. nov. (FT135W=GDMCC 1.1745=KACC 21659) and sp. nov. (FT92W=GDMCC 1.1682=KACC 21474) are proposed.
从中国亚热带溪流中分离出五株革兰氏阴性、过氧化氢酶和氧化酶阳性、杆状且具运动性的菌株（FT50W、FT80W、FT92W、FT94W和FT135W）。基于16S rRNA基因序列的比较表明，在系统发育树中，菌株FT50W、FT94W和FT135W与菌株Sac - 22亲缘关系最近，菌株FT80W和FT92W分别与菌株DCY83亲缘关系最近。菌株FT50W、FT80W、FT92W、FT94W和FT135W的G + C含量分别为63.3%、62.4%、62.8%、63.8%和60.8%。基于92个串联核心基因构建的系统发育基因组树表明，菌株FT50W、FT80W、FT94W和FT135W与某属的物种聚集在一起，但菌株FT92W和KCTC 42409位于另一属的分支中。计算得到的菌株FT50W、FT80W、FT92W、FT94W、FT135W与相关菌株之间的成对平均核苷酸同一性（ANI）和数字DNA - DNA杂交（dDDH）值在75.6 - 87.8%和20.3 - 33.8%范围内，除了菌株FT50W和FT94W之间的值分别为98.7%和89.2%。这五株菌株的呼吸醌为Q - 8。主要脂肪酸为C 7、C、C ω7和C。极性脂质包括磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的磷脂。考虑到在系统发育基因组树中与某属物种的明显系统发育关系，将其转移到另一属作为新组合是合理的。综合系统发育基因组分析结果、ANI和dDDH数据以及一系列生理生化特征，菌株FT50W和FT94W应属于同一物种，并与菌株FT80W和FT135W一起归入某属，菌株FT92W应归入另一属，为此提出新物种名称某新种（模式菌株FT50W = GDMCC 1.1674 = KACC 21466）、某新种（FT80W = GDMCC 1.1678 = KACC 21470）、某新种（FT135W = GDMCC 1.1745 = KACC 21659）和某新种（FT92W = GDMCC 1.1682 = KACC 21474）。
