Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006090.
Two Gram-stain-negative, catalase- and oxidase-positive, aerobic non-motile and motile rod bacteria, strains MSW6 and RSW2, were isolated from surface seawater. Strain MSW6 optimally grew at 20 °C, pH 7.0 and 3 % NaCl, while strain RSW2 optimally grew at 25 °C, pH 7.0-8.0 and 2 % NaCl. Strain MSW6 possessed menaquinone-6 as the major respiratory quinone, and its major fatty acids were iso-C G, iso-C and iso-C 3-OH. The major polar lipid identified in strain MSW6 was phosphatidylethanolamine (PE). On the other hand, strain RSW2 had ubiquinone-8 as the predominant respiratory quinone, and its major fatty acids consisted of summed feature 3 (C 7 and/or C 6), summed feature 8 (C 7 and/or C 6) and C. The major polar lipids identified in strain RSW2 were PE and phosphatidylglycerol. As the sole respiratory quinone, strain MSW6 possessed menaquinone-6, while strain RSW2 had ubiquinone-8. The DNA G+C contents of strains MSW6 and RSW2 were 31.9 and 43.4 mol%, respectively. Phylogenetic analyses based on 16S rRNA and core gene sequences showed that strain MSW6 formed a phylogenic lineage with KOPRI 13649, while strain RSW2 formed a phylogenic lineage with KMM 3633. Strain MSW6 shared 97.9 % 16S rRNA gene sequence similarity and 80.7 % average nucleotide identity (ANI) ith KOPRI 13649, and strain RSW2 shared 99.1 % 16S rRNA gene sequence similarity and 93.1 % ANI with KMM 3633. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strains MSW6 and RSW2 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of is MSW6 (=KACC 22338=JCM 35022) and the type strain of is RSW2 (=KACC 22716=JCM 35550).
从表层海水中分离到两株革兰氏阴性、接触酶和氧化酶阳性、需氧非运动和运动杆状细菌,分别为 MSW6 株和 RSW2 株。MSW6 株在 20°C、pH7.0 和 3%NaCl 下最佳生长,而 RSW2 株在 25°C、pH7.0-8.0 和 2%NaCl 下最佳生长。MSW6 株的主要呼吸醌为menaquinone-6,其主要脂肪酸为iso-C G、iso-C 和 iso-C 3-OH。MSW6 株中鉴定出的主要极性脂是磷脂酰乙醇胺(PE)。另一方面,RSW2 株以 ubiquinone-8 作为主要呼吸醌,其主要脂肪酸由总和特征 3(C 7 和/或 C 6)、总和特征 8(C 7 和/或 C 6)和 C 组成。RSW2 株中鉴定出的主要极性脂是 PE 和磷脂酰甘油。作为唯一的呼吸醌,MSW6 株含有menaquinone-6,而 RSW2 株含有 ubiquinone-8。MSW6 株和 RSW2 株的 DNA G+C 含量分别为 31.9%和 43.4%mol。基于 16S rRNA 和核心基因序列的系统发育分析表明,MSW6 株与 KOPRI 13649 形成一个系统发育谱系,而 RSW2 株与 KMM 3633 形成一个系统发育谱系。MSW6 株与 KOPRI 13649 的 16S rRNA 基因序列相似性为 97.9%,平均核苷酸同一性(ANI)为 80.7%,与 RSW2 株与 KMM 3633 的 16S rRNA 基因序列相似性为 99.1%,ANI 为 93.1%。基于表型、化学分类和系统发育分析的结果,MSW6 株和 RSW2 株分别代表 和属的新种,分别提议命名为 sp. nov. 和 sp. nov.。的模式菌株是 MSW6(=KACC 22338=JCM 35022),的模式菌株是 RSW2(=KACC 22716=JCM 35550)。