Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Ruta 8 km 18, Montevideo, 13000, Uruguay.
Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, 24230-340, Brazil.
Theriogenology. 2022 Oct 1;191:96-101. doi: 10.1016/j.theriogenology.2022.08.008. Epub 2022 Aug 9.
This study aimed to identify the possible advantages of administering butorphanol (BUT), associated with ketamine/xylazine (KX) sedation or not, for semen collection using electroejaculation (EE) in rams. Fifteen rams received four treatments in a cross-over design using a 2 × 2 factorial arrangement. The treatments were 1) control; 2) received KX; 3) received BUT; and 4) received both treatments. The responses to the procedures regarding heart rate, respiratory frequency, and rectal temperature were recorded, glucose and creatine kinase were measured, and semen quality was determined. More pulses were required when BUT was administered (P = 0.0067), but rams vocalized fewer times (P = 0.046). The administration of KX also tended to reduce the respiratory frequency (P = 0.068) and rectal temperature (P = 0.089), with no other effects. The administration of BUT reduced the heart rate (P < 0.0001), and there was an interactive effect between the administration of BUT and time on the heart rate (P < 0.0001), respiratory frequency (P = 0.01), and rectal temperature (P = 0.047). The administration of BUT reduced the heart rate increase immediately after EE (P < 0.0001). The respiratory frequency following EE was greater when BUT was administered (P < 0.0001), but the administration of BUT reduced the rectal temperature at the same time (P = 0.002). Glucose concentration was greater when BUT was administered (P < 0.0001). The only significant effect on the ejaculate characteristics was from the interaction between KX and BUT on sperm concentration (P = 0.004). The administration of KX alone increased sperm concentration (P = 0.035), but when BUT was added to KX, sperm concentration decreased (P = 0.002). Moreover, sperm concentration was greater when only BUT was administered than when KX and BUT were administered simultaneously (P = 0.037). The addition of BUT markedly decreased most negative responses more effectively than sedation with KX alone. In conclusion, BUT appears to be an interesting alternative to reduce the welfare concerns raised by the use of EE in rams; however, its possible effects on the ejaculate characteristics when associated with other anesthesia/sedation drugs require further study.
本研究旨在确定在使用电刺激采精（EE）对绵羊进行精液采集时，联合或不联合使用丁丙诺啡（BUT）和氯胺酮/二甲噻嗪（KX）镇静的可能优势。15 只绵羊采用交叉设计 2×2 析因设计接受了四种处理。处理 1）对照组；2）接受 KX；3）接受 BUT；4）同时接受两种处理。记录了心率、呼吸频率和直肠温度对这些程序的反应，测量了葡萄糖和肌酸激酶，确定了精液质量。给予 BUT 时需要更多的脉冲（P=0.0067），但羊的发声次数更少（P=0.046）。KX 的给药也倾向于降低呼吸频率（P=0.068）和直肠温度（P=0.089），但没有其他影响。BUT 的给药降低了心率（P<0.0001），BUT 的给药与时间对心率（P<0.0001）、呼吸频率（P=0.01）和直肠温度（P=0.047）的交互作用有影响。BUT 的给药即刻降低了 EE 后的心率增加（P<0.0001）。BUT 给药后，EE 后的呼吸频率更大（P<0.0001），但同时 BUT 的给药降低了直肠温度（P=0.002）。葡萄糖浓度在给予 BUT 时更高（P<0.0001）。对精液特性唯一有显著影响的是 KX 和 BUT 之间的相互作用对精子浓度的影响（P=0.004）。单独使用 KX 增加了精子浓度（P=0.035），但当 BUT 与 KX 一起使用时，精子浓度降低（P=0.002）。此外，与同时使用 KX 和 BUT 相比，仅给予 BUT 时精子浓度更高（P=0.037）。与单独使用 KX 镇静相比，BUT 给药明显更有效地降低了大多数负面反应。总之，BUT 似乎是一种减少电刺激采精对绵羊福利影响的有趣选择；然而，当与其他麻醉/镇静药物联合使用时，它对精液特性的可能影响需要进一步研究。
