Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Ruta 8 km 18, Montevideo 13000, Uruguay; Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Zacatepec, Carretera Zacatepec-Galeana km 0.05, Zacatepec, Morelos 62780, Mexico.
Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Ruta 8 km 18, Montevideo 13000, Uruguay.
Anim Reprod Sci. 2024 Sep;268:107565. doi: 10.1016/j.anireprosci.2024.107565. Epub 2024 Jul 19.
The aim of this study was to determine whether brushing rams before and during electroejaculation (EE) reduces their stress response and improves the characteristics of the ejaculate. A single person brushed each ram for 5 min daily, for 15 days, in an individual pen. Semen was collected from five rams brushed before and during EE by the same brusher, while the other five were electroejaculated without being brushed. The treatments were exchanged three days later, so semen was collected from all the rams with both treatments. Brushing increased mass motility (P = 0.05), and curvilinear (P = 0.001), linear (P = 0.02), and average path (P = 0.01) velocities of sperm, as well as the average amplitude of lateral displacement of the sperm head (P = 0.05), and tended to increase sperm concentration (P = 0.09). Brushing tended to reduce the cortisol concentration (P = 0.06) and the duration of head movements when 2 V pulse series V was applied (P = 0.1). Brushing increased creatine kinase concentration (P = 0.04) and tended to increase rectal (P = 0.06) and maximum eye surface temperatures (P = 0.1), total time, and number of electrical pulses administered (P = 0.07 for both variables), as well as the sum of pulses per voltage applied during EE (P = 0.06). In rams accustomed to being brushed by the same person, brushing them before and during EE improved semen quality, with slight changes in the stress responses.
本研究旨在确定在电刺激采精（EE）前和期间给公羊刷毛是否会降低其应激反应并改善精液特征。一个人每天在一个单独的围栏中给每只公羊刷毛 5 分钟，共 15 天。在同一个刷毛者给五头公羊在电刺激采精前和期间进行刷毛，而另外五头公羊则在不刷毛的情况下进行电刺激采精。三天后，处理方法进行了交换，因此所有公羊都接受了这两种处理方法的精液采集。刷毛增加了质量运动性（P=0.05），以及曲线（P=0.001）、直线（P=0.02）和平均路径（P=0.01）速度的精子，以及精子头部侧向位移的平均幅度（P=0.05），并倾向于增加精子浓度（P=0.09）。刷毛有降低皮质醇浓度的趋势（P=0.06）和应用 2V 脉冲系列 V 时头部运动的持续时间（P=0.1）。刷毛增加了肌酸激酶浓度（P=0.04），并倾向于增加直肠（P=0.06）和最大眼表面温度（P=0.1）、总时间和施加的电脉冲数（两者均为 P=0.07），以及在 EE 期间施加的每伏脉冲数（P=0.06）。在习惯于被同一个人刷毛的公羊中，在电刺激采精前和期间给它们刷毛可提高精液质量，同时应激反应略有变化。
