Bioengineering College, Chongqing University, Chongqing 400044, China; State Key Laboratory of Trauma, Burns and Combined Injury, Department of Wound Infection and Drug, Daping Hospital, Army Medical University, Chongqing 400042, China.
Bioengineering College, Chongqing University, Chongqing 400044, China.
Plant Sci. 2022 Oct;323:111417. doi: 10.1016/j.plantsci.2022.111417. Epub 2022 Aug 13.
N-methyladenosine (mA), the most abundant and common modification on eukaryotic mRNA, plays crucial roles in multiple biological processes through controlling endogenous gene activity in organisms. The mA reader specifically recognizes the mA mark to mediate the regulation of mA on mRNA, and determines the fate of its target mRNA. In plants, the currently confirmed mA readers are YTH (YT521B homology) domain-containing proteins. We previously reported that tomato contains 9 YTH genes, of which SlYTH1 has the strongest expression. The present study reports the functional characterization of SlYTH1 in tomato. SlYTH1 mutants generated via CRISPR/Cas9 technology exhibited pleiotropic phenotypes, including low seed germination rate, shortened seedling root, retarded plant growth and development during vegetative development, and elongated and longitudinally flattened fruit with reduced the locule number. SlYTH1 knockout reduced GA content and downregulated the expression of related genes in gibberellin biosynthesis pathway. Moreover, exogenous GA application could partially restore the phenotypic defects caused by SlYTH1 mutations. SlYTH1 knockout could alleviate the inhibition of seedling root elongation by exogenous GA application at relatively low concentration. These facts indicated SlYTH1 is involved in regulating gibberellin biosynthesis and plays important roles in multiple physiological processes in tomato.
N6-甲基腺苷（m6A）是真核生物 mRNA 上最丰富、最常见的修饰，通过控制生物体内内源性基因活性，在多种生物学过程中发挥关键作用。m6A 阅读器特异性识别 m6A 标记，介导 m6A 对 mRNA 的调控，并决定其靶 mRNA 的命运。在植物中，目前已确认的 m6A 阅读器是 YTH（YT521B 同源）结构域蛋白。我们之前报道过番茄含有 9 个 YTH 基因，其中 SlYTH1 的表达最强。本研究报告了番茄 SlYTH1 的功能特征。通过 CRISPR/Cas9 技术生成的 SlYTH1 突变体表现出多种表型，包括种子发芽率低、幼苗根缩短、营养生长发育迟缓、果实伸长和纵向扁平、心室数量减少。SlYTH1 敲除降低了赤霉素含量，并下调了赤霉素生物合成途径中相关基因的表达。此外，外源 GA 的应用可以部分恢复 SlYTH1 突变引起的表型缺陷。SlYTH1 敲除可以减轻外源 GA 应用在相对较低浓度下对幼苗根伸长的抑制作用。这些事实表明，SlYTH1 参与调节赤霉素的生物合成，并在番茄的多个生理过程中发挥重要作用。
