Department of Plant Pathology, Zhejiang Agriculture and Forest University, Hangzhou 311300, China.
Department of Plant Pathology, Zhejiang Agriculture and Forest University, Hangzhou 311300, China.
Pestic Biochem Physiol. 2022 Aug;186:105169. doi: 10.1016/j.pestbp.2022.105169. Epub 2022 Jul 6.
Emergence and development of resistance to 14α-demethylase inhibitors (DMIs) have become a critical issue in both agriculture and medical fields. Mefentrifluconazole, the first isopropanol triazole fungicide belonging to a new subclass of DMIs, has been proposed to show high activity, minimal adverse side effects, and inconsistent cross resistance with other DMIs due to its high structural flexibility. In this study, mefentrifluconazole showed disparate inhibitory activity against the mycelium growth of seven tested Fusarium species. The most sensitive species included F. oxysporum, F. proliferatum, F. commuae, and F. fujikuroi, followed by F. equiseti and F. graminearum, while F. solani was most insensitive. Consistently, mefentrifluconazole presented the strongest inhibiting effects on conidium germination, cell membrane integrity, and ergosterol biosynthesis in F. fujikuroi, followed by F. graminearum, while F. solani ranked last. Further results indicated that all F. fujikuroi isolates causing rice bakanae disease (RBD) were sensitive to mefentrifluconazole regardless of their resistance to prochloraz, tebuconazole, carbendazim, and phenamacril. Additionally, the inoculation tests found that mefentrifluconazole presented a better protective efficacy on rice seedlings when applied 12 h before the F. fujikuroi inoculation, compared to applied 12 h post the inoculation. Overall, this study demonstrated the various bioactivity of mefentrifluconazole combating Fusarium spp. and put new insights into RBD management as well as the applications of DMIs.
14α-脱甲基酶抑制剂（DMIs）的抗性的出现和发展已成为农业和医学领域的一个关键问题。由于其结构高度灵活，丙硫菌唑是第一个异丙醇三唑类杀菌剂，属于 DMIs 的一个新子类，被提议具有高活性、最小的不良反应和与其他 DMIs 不一致的交叉抗性。在这项研究中，丙硫菌唑对七种测试的镰刀菌属真菌的菌丝生长表现出不同的抑制活性。最敏感的物种包括尖孢镰刀菌、层出镰刀菌、禾谷镰刀菌和藤仓镰刀菌，其次是旋孢腔菌和禾谷镰刀菌，而茄病镰刀菌最不敏感。一致地，丙硫菌唑对藤仓镰刀菌的分生孢子萌发、细胞膜完整性和麦角固醇生物合成表现出最强的抑制作用，其次是禾谷镰刀菌，而茄病镰刀菌排名最后。进一步的结果表明，引起水稻恶苗病（RBD）的所有藤仓镰刀菌分离株均对丙硫菌唑敏感，无论它们对丙环唑、戊唑醇、多菌灵和苯酰胺的抗性如何。此外，接种试验发现，与接种后 12 小时施药相比，在藤仓镰刀菌接种前 12 小时施药时，丙硫菌唑对水稻幼苗的保护效果更好。总体而言，这项研究表明了丙硫菌唑对镰刀菌属的各种生物活性，并为 RBD 管理以及 DMIs 的应用提供了新的见解。
