CRISPR 升级可解锁弓形虫基因功能。

A CRISPR upgrade unlocks Toxoplasma gene function.

机构信息

The Division of Infectious Diseases and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria, Australia; Department of Medical Biology, The University of Melbourne, Parkville, Melbourne, Victoria, Australia.

出版信息

Trends Parasitol. 2022 Oct;38(10):826-828. doi: 10.1016/j.pt.2022.07.007. Epub 2022 Aug 13.

DOI:10.1016/j.pt.2022.07.007

PMID:35973902

Abstract

Forward genetic screens are invaluable in describing gene function. CRISPR has reinvigorated phenotypic screens in Toxoplasma - a model apicomplexan parasite. Two recent papers by Smith et al. and Li et al. take the next big leap in performing forward genetic screens in Toxoplasma by combining conditional gene regulation with CRISPR.

摘要

正向遗传学筛选在描述基因功能方面具有不可估量的价值。CRISPR 技术重新激发了弓形虫（一种模式顶复门寄生虫）表型筛选的活力。Smith 等人和 Li 等人的两篇最新论文通过将条件基因调控与 CRISPR 相结合，在弓形虫的正向遗传学筛选方面迈出了一大步。