C57BL/6 小鼠和 SD 大鼠掌侧皮肤中汗腺和毛囊的差异分布和遗传决定。
Differential distribution and genetic determination of eccrine sweat glands and hair follicles in the volar skin of C57BL/6 mice and SD rats.
机构信息
Jinzhou Medical University Graduate Training Base, Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China.
Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China.
出版信息
BMC Vet Res. 2022 Aug 16;18(1):316. doi: 10.1186/s12917-022-03416-z.
BACKGROUND
Eccrine sweat glands (ESGs) and hair follicles (HFs) are the prominent skin appendages regulating human body temperature. C57BL/6 mice and Sprague-Dawley (SD) rats are the most commonly used model animals for studying ESGs and HFs. Previous studies have shown the distribution of ESGs and HFs in volar hindfeet of C57BL/6 mice, but there are few or no reports on the distribution of ESGs and HFs in volar forefeet of C57BL/6 mice and volar feet of SD rats. Here, we investigated the differential distribution and genetic determination of ESGs and HFs in the volar skin of C57BL/6 mice and SD rats through gross observation, iodine-starch sweat test, double staining with Nile Blue A and Oil Red O, hematoxylin and eosin (HE) staining, double immunofluorescence staining of LIM Homeobox 2 (LHX2)/Na-K-ATPase α1(NKA) or LHX2/Na-K-2Cl cotransporter 1 (NKCC1), and qRT-PCR detection of ESG-related gene Engrailed 1 (En1) and HF-related gene LHX2.
RESULTS
The results showed ESGs but no HFs in the footpads of C57BL/6 mice and SD rats, both ESGs and HFs in the inter-footpads (IFPs) of C57BL/6 mice, and neither ESGs nor HFs in the IFPs of SD rats. The relative quantitative change in En1 was consistent with the differential distribution of ESGs, and the relative quantitative change of LHX2 was consistent with the differential distribution of HFs.
CONCLUSION
C57BL/6 mice and SD rats had their own characteristics in the distribution of ESGs and HFs in the volar skin, and researchers should choose mice or rats, and even forefeet or hindfeet as their research object according to different purposes. The study provides a basis for selection of optimal animal models to study development, wound healing and regeneration of skin appendages.
背景
小汗腺（ESG）和毛囊（HF）是调节人体体温的主要皮肤附属物。C57BL/6 小鼠和 Sprague-Dawley（SD）大鼠是研究 ESG 和 HF 的最常用模型动物。先前的研究表明 C57BL/6 小鼠掌跖侧后足的 ESG 和 HF 分布情况，但关于 C57BL/6 小鼠掌跖侧前足和 SD 大鼠掌跖侧皮肤的 ESG 和 HF 分布情况的报道较少或没有。 在这里，我们通过大体观察、碘淀粉汗试验、尼罗蓝 A 和油红 O 双重染色、苏木精和伊红（HE）染色、LIM 同源盒 2（LHX2）/Na-K-ATPase α1（NKA）或 LHX2/Na-K-2Cl 协同转运蛋白 1（NKCC1）的双重免疫荧光染色以及 ESG 相关基因 Engrailed 1（En1）和 HF 相关基因 LHX2 的 qRT-PCR 检测，研究了 C57BL/6 小鼠和 SD 大鼠掌侧皮肤中 ESG 和 HF 的差异分布和遗传决定因素。
结果
结果显示 C57BL/6 小鼠和 SD 大鼠的足底仅有 ESG 而无 HF，C57BL/6 小鼠的足垫（IFP）既有 ESG 又有 HF，而 SD 大鼠的 IFP 中则既无 ESG 也无 HF。En1 的相对定量变化与 ESG 的差异分布一致，而 LHX2 的相对定量变化与 HF 的差异分布一致。
结论
C57BL/6 小鼠和 SD 大鼠掌侧皮肤 ESG 和 HF 的分布存在自身特点，研究人员应根据不同目的选择小鼠或大鼠，甚至前足或后足作为研究对象。本研究为选择最佳动物模型研究皮肤附属物的发育、创伤愈合和再生提供了依据。
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