Histology and Embryology Department, Naval Medical University, 800, Xiangyin Road, 200433, Shanghai, People's Republic of China.
Shanghai Key Laboratory of Cell Engineering, Naval Medical University, 800, Xiangyin Road, 200433, Shanghai, People's Republic of China.
Mol Cancer. 2022 Aug 16;21(1):164. doi: 10.1186/s12943-022-01612-x.
Developing a strategy to specifically kill cancer cells without inducing obvious damage to normal cells may be of great clinical significance for cancer treatment. In the present study, we developed a new precise personalized strategy named "i-CRISPR" for cancer treatment through adding DNA damage repair inhibitors(i) and inducing cancer cell-specific DNA double strand breaks by CRISPR. Through in vitro and in vivo experiments, we confirmed the efficacy of this strategy in multiple cancer models and revealed the mechanism of cell death. Our strategy might provide a novel concept for precise cancer therapy.
开发一种专门杀死癌细胞而不引起正常细胞明显损伤的策略，可能对癌症治疗具有重要的临床意义。本研究通过添加 DNA 损伤修复抑制剂(i)和 CRISPR 诱导癌细胞特异性 DNA 双链断裂，开发了一种名为“i-CRISPR”的新型精确个体化癌症治疗策略。通过体外和体内实验，我们在多种癌症模型中证实了该策略的疗效，并揭示了细胞死亡的机制。我们的策略可能为精确的癌症治疗提供了一个新的概念。
