Kawabata Fuminori, Murayama Kana, Yoshida Yuta, Liang Ruojun, Nishimura Shotaro, Tabata Shoji
Laboratory of Functional Anatomy, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.
Physiology of Domestic Animals, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan.
J Poult Sci. 2022 Jul 25;59(3):286-290. doi: 10.2141/jpsa.0210132.
The pungency induced by spices and herbs plays an important role in food choice and appetite, and it is suggested that adding spices and herbs to feed as natural alternatives to antibiotics has beneficial effects in poultry farming. However, our knowledge of the chemosensory perception of herbal compounds in chickens is limited. Transient receptor potential ankyrin 1 (TRPA1) is involved in the sensory perception of various herbal compounds. Here, we performed calcium imaging and electrophysiological analyses using cells transiently expressing chicken TRPA1 (cTRPA1) and identified two novel cTRPA1 ligands-eugenol and thymol. In a behavioral assay, chickens responded to cTRPA1 ligands, including eugenol, thymol, cinnamaldehyde, carvacrol, and allyl isothiocyanate. These results provide evidence that chickens have a functional TRPA1 channel and chemosensory perception of various herbal compounds.
香料和药草所产生的辛辣味在食物选择和食欲方面起着重要作用，并且有人提出在饲料中添加香料和药草作为抗生素的天然替代品在家禽养殖中具有有益效果。然而，我们对鸡对草药化合物的化学感觉认知有限。瞬时受体电位锚蛋白1（TRPA1）参与各种草药化合物的感觉认知。在此，我们使用瞬时表达鸡TRPA1（cTRPA1）的细胞进行了钙成像和电生理分析，并鉴定出两种新型cTRPA1配体——丁香酚和百里酚。在行为试验中，鸡对cTRPA1配体有反应，包括丁香酚、百里酚、肉桂醛、香芹酚和异硫氰酸烯丙酯。这些结果提供了证据，证明鸡具有功能性TRPA1通道以及对各种草药化合物的化学感觉认知。
