Cai Chun-He, Wang He-Li, Man Ruo-Jun
School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Rd., Beijing 100083, PR China; Beijing Kaiheyingran Consulting Co., Ltd., F-101, Fuliaidingbao, Baijiazhuang No.1, Chaoyang Dist, Beijing 100020, China; Nanjing University, School of Life Science, Xianlin Campus, No.163, Xianlin Rd, 210093 Nanjing, China.
School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Rd., Beijing 100083, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jul 5;255:119729. doi: 10.1016/j.saa.2021.119729. Epub 2021 Mar 22.
Physiologically, Fe(III) and Fe(II) is the most important redox pairs in a variety of biological and environmental procedures with its capability of transition. The detection of physiological iron, especially Fe(II), has become the recent research focus of investigations on revealing the mechanism of iron-related metabolism. In this work, we exploited a novel quinoline-derived fluorescent probe, YTP, for the detection of Fe(II). It could monitor the level of Fe(II) with a linear range of 0-2.0 equivalent and the detection limit of 0.16 µM. High selectivity from other analytes including Fe(III) and steadiness for over 24 h confirmed the practicability of YTP. YTP was further applied in real buffer systems and in cellular imaging. The probe could achieve the semi-quantitative monitoring of Fe(II) in living cells. This work provided a potential implement for the detection of Fe(II), and raised important information for further researches on the redox pairs of iron, in mechanism and in practice.
在生理学上，Fe(III)和Fe(II)凭借其转变能力，是各种生物和环境过程中最重要的氧化还原对。生理铁尤其是Fe(II)的检测，已成为近期揭示铁相关代谢机制研究的焦点。在这项工作中，我们开发了一种新型喹啉衍生荧光探针YTP用于检测Fe(II)。它能够监测Fe(II)水平，线性范围为0至2.0当量，检测限为0.16 μM。对包括Fe(III)在内的其他分析物具有高选择性以及超过24小时的稳定性证实了YTP的实用性。YTP进一步应用于实际缓冲系统和细胞成像。该探针能够实现活细胞中Fe(II)的半定量监测。这项工作为Fe(II)的检测提供了一种潜在工具，并在机制和实践方面为铁氧化还原对的进一步研究提供了重要信息。
