Yang Yi, Jiang Yiyang, Jiang Huijun, Qi Xiuxiu, Zhu Li, Ju Yichun, Wang Yuan, Peng Yan, Mei Yang, Khant Zaw Hein, Smruti Mohanty Shreya, Abishek B, Zhou Zhijie, Tang Jie, Zhang Zhenqin
Changzhou Vocational Institute of Engineering, Changzhou 213164, China.
The First Affiliated Hospital, Nanjing Medical University, Nanjing 211166, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 5;324:124993. doi: 10.1016/j.saa.2024.124993. Epub 2024 Aug 15.
BODIPY-based chemosensors are widely used owing to merits like good selectivity, high fluorescence quantum yield, and excellent optical stability. As such, a pH-switchable hydrophilic fluorescent probe, BODIPY-PY-(SONa), was developed for detection of Fe ion in aqueous solutions. BODIPY-PY-(SONa) revealed strong fluorescence intensity and was responsive to pH value in the range of 6.59-1.96. Additionally, BODIPY-PY-(SONa) showed good selectivity and sensitivity towards Fe. A good linear relationship for Fe detection was obtained from 0.0 μM to 50.0 μM with low detecting limit of 6.34 nM at pH 6.59 and 2.36 nM at pH 4.32, respectively. The response to pH and detection of Fe induced obvious multicolor changes. BODIPY-PY-(SONa) can also be utilized to quantitatively detect Fe in real water sample. Different mechanisms of Fe detection at investigated pH values were unraveled through relativistic density functional theory (DFT) calculations in BODIPY-PY-(SONa) and experiments of coexisting cations, anions and molecules. These results enabled us to gain a deeper understanding of the interactions between BODIPY-PY-(SONa) and Fe and provide valuable fundamental information for design of efficient multicolor chemosensors for Fe as well.
基于硼二吡咯的化学传感器因其具有良好的选择性、高荧光量子产率和出色的光学稳定性等优点而被广泛使用。因此，开发了一种pH可切换的亲水性荧光探针BODIPY-PY-(SONa)，用于检测水溶液中的铁离子。BODIPY-PY-(SONa)显示出很强的荧光强度，并且在6.59 - 1.96的pH值范围内对pH值有响应。此外，BODIPY-PY-(SONa)对铁表现出良好的选择性和灵敏度。在0.0 μM至50.0 μM范围内获得了良好的铁检测线性关系，在pH 6.59时检测限低至6.34 nM，在pH 4.32时检测限为2.36 nM。对pH的响应和铁的检测引起了明显的多色变化。BODIPY-PY-(SONa)还可用于定量检测实际水样中的铁。通过对BODIPY-PY-(SONa)进行相对论密度泛函理论(DFT)计算以及共存阳离子、阴离子和分子的实验，揭示了在所研究的pH值下铁检测的不同机制。这些结果使我们能够更深入地了解BODIPY-PY-(SONa)与铁之间的相互作用，并为设计高效的铁多色化学传感器提供有价值的基础信息。
