Clin Lab. 2022 Aug 1;68(8). doi: 10.7754/Clin.Lab.2021.211005.
To explore the effects of different contents and types of hand sanitizers on the complete blood count and leukocyte differential count.
EDTA anticoagulant whole blood samples of healthy individuals were selected, and were treated with 75% alcohol, liquid hand sanitizer, and gel hand sanitizer. The samples were detected by the Sysmex automatic blood analyzer, and the capillary blood smear was prepared. The appearance of cells was examined under the microscope.
The absolute lymphocyte count, MCV, MCHC, and HGB were significantly increased, while PLT and MCH were significantly decreased after adding 1 µL 75% alcohol (p < 0.05). By adding 1 µL liquid hand sanitizer, the absolute counts of lymphocyte, neutrophil and basophilic, MCHC, HGB, and PLT were significantly increased (p < 0.05). The results of WBC, MCHC and HGB were significantly increased when 1 µL gel hand sanitizer was added. The classification of leukocytes was obviously abnormal and could not be detected by the instrument. The effect of gel hand sanitizer on WBC was time-dependent. Pyknotic, karyorrhexis, and karyolysis of nuclei were observed under microscope.
Three kinds of hand sanitizers had effects on complete blood counts and leukocyte differential counts, in particular, gel hand sanitizer has the greatest impact on the results of tests. Therefore, gel hand sanitizer is not suitable for hand disinfection of operators before the collection of capillary blood samples.
探讨不同成分和类型的手消毒剂对全血细胞计数和白细胞分类计数的影响。
选取健康个体的 EDTA 抗凝全血样本，分别用 75%酒精、液体手消毒剂和凝胶手消毒剂处理。用 Sysmex 全自动血液分析仪进行检测，同时制作毛细血管血涂片，在显微镜下观察细胞形态。
加入 1µL 75%酒精后，绝对淋巴细胞计数、MCV、MCHC 和 HGB 显著升高，PLT 和 MCH 显著降低（p＜0.05）。加入 1µL 液体手消毒剂后，淋巴细胞、中性粒细胞、嗜碱性粒细胞、MCHC、HGB 和 PLT 的绝对计数显著升高（p＜0.05）。加入 1µL 凝胶手消毒剂后，WBC、MCHC 和 HGB 的结果显著升高。白细胞分类明显异常，仪器无法检测。凝胶手消毒剂对 WBC 的影响具有时间依赖性。显微镜下观察到细胞核固缩、核碎裂和核溶解。
三种手消毒剂对全血细胞计数和白细胞分类计数有影响，尤其是凝胶手消毒剂对检测结果的影响最大。因此，凝胶手消毒剂不适合在采集毛细血管血样前对手部进行消毒。
