Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA.
Program for Computational and Systems Biology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Open Biol. 2022 Aug;12(8):220180. doi: 10.1098/rsob.220180. Epub 2022 Aug 17.
Bacterial proteases are a promising post-translational regulation strategy in synthetic circuits because they recognize specific amino acid degradation tags (degrons) that can be fine-tuned to modulate the degradation levels of tagged proteins. For this reason, recent efforts have been made in the search for new degrons. Here we review the up-to-date applications of degradation tags for circuit engineering in bacteria. In particular, we pay special attention to the effects of degradation bottlenecks in synthetic oscillators and introduce mathematical approaches to study queueing that enable the quantitative modelling of proteolytic queues.
细菌蛋白酶是合成回路中一种很有前途的翻译后调控策略，因为它们可以识别特定的氨基酸降解标签（degrons），这些标签可以被精细地调整以调节标记蛋白的降解水平。出于这个原因，最近人们一直在寻找新的 degrons。在这里，我们回顾了降解标签在细菌回路工程中的最新应用。特别是，我们特别关注在合成振荡器中降解瓶颈的影响，并介绍了用于研究队列的数学方法，从而能够对蛋白水解队列进行定量建模。
