Department of Periodontics, SRM Dental College, Chennai, India.
Genet Test Mol Biomarkers. 2022 Jul-Aug;26(7-8):391-397. doi: 10.1089/gtmb.2022.0018. Epub 2022 Aug 17.
Caspase-3 activation is central to apoptosis and is involved in periodontal disease pathogenesis. Thus, gene polymorphisms in a population may influence the severity of periodontal disease. In this study we examined the association of the CASP3 alleles and genotypes with periodontitis using cohorts of periodontally diseased and healthy South Indian subjects of Tamil ethnicity. Allele specific polymerase chain reaction genotyping were performed to identify gene polymorphism in 145 periodontally healthy subjects and 145 periodontitis patients. The associations between gene polymorphism frequencies and the risk of periodontitis were analyzed by the Pearson chi-square test, the Mann Whitney test, test, logistic regression and assessed by odds ratio (ORs) and 95% confidence interval (95% CIs). The statistical test results demonstrated a significant difference in genotype distribution between the two groups ( < 0.001) with the variant GG genotype associated with a significantly increased risk for periodontitis (OR = 3.55, 95% CI = 1.89-6.68, < 0.001). Furthermore, all clinical parameters were highly significantly correlated with the genotypes in periodontitis subjects ( < 0.05). The present study suggests that the GG genotype of CASP3 is associated with the increased periodontitis risk in South Indians of Tamil ethnicity. : (CTRI/2021/03/032412).
半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）的激活是细胞凋亡的核心，并且参与牙周病的发病机制。因此，人群中的基因多态性可能会影响牙周病的严重程度。在这项研究中，我们使用泰米尔裔的南印度患有牙周病和健康的队列检查了 CASP3 等位基因和基因型与牙周炎的关系。通过等位基因特异性聚合酶链反应基因分型，对 145 名牙周健康的受试者和 145 名牙周炎患者的基因多态性进行了鉴定。通过皮尔逊卡方检验、曼惠特尼 U 检验、检验、逻辑回归分析以及优势比（OR）和 95%置信区间（95%CI）评估，分析了基因多态性频率与牙周炎风险之间的关联。统计检验结果表明，两组之间的基因型分布存在显著差异（ < 0.001），其中变体 GG 基因型与牙周炎的风险显著增加相关（OR=3.55，95%CI=1.89-6.68， < 0.001）。此外，所有临床参数与牙周炎患者的基因型高度相关（ < 0.05）。本研究表明，在泰米尔裔的南印度人群中，Caspase-3 的 GG 基因型与牙周炎风险增加有关。注册号：CTRI/2021/03/032412。
