Kaarthikeyan Gurumoorthy, Jayakumar Nadathur Doraisamy, Padmalatha Ogoti, Sheeja Varghese, Sankari Malaippan, Anandan Balakrisnan
Department of Periodontics, Saveetha Dental College, 162 P.H Road, Velappanchavadi, Chennai 600 077, India.
Indian J Dent Res. 2009 Jan-Mar;20(1):37-40. doi: 10.4103/0970-9290.49061.
The aim of this study was to determine the association between IL-1B (+3954) gene polymorphism and chronic periodontitis in a sample of the south Indian population.
This study employed a cross-sectional design involving individuals from the state of Tamil Nadu in the southern part of India.
Genomic DNA was obtained from the white blood cells of 30 patients with chronic periodontitis (18 males and 12 females) and 31 healthy controls (20 males and 11 females). The age of the subjects ranged from 30 to 55 years old and all were non smokers. DNA was amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus +3954 of IL-1beta gene and analyzed by 3% agarose gel electrophoresis.
A Chi-square test was used to determine the genotype distribution between the groups and the relative risk was estimated with a 95% confidence interval.
The chronic periodontitis group displayed a higher percentage of T allele, even though it was not statistically significant. The relative risk analysis between genotypes showed that the risk was higher for the CT genotype compared with the CC genotype and the risk was significant. In conclusion, our data suggested that there was no significant association between IL-1beta (+3954) gene polymorphism and chronic periodontitis in the south Indian population.
本研究旨在确定印度南部人群样本中白细胞介素-1β（IL-1B，+3954）基因多态性与慢性牙周炎之间的关联。
本研究采用横断面设计，涉及印度南部泰米尔纳德邦的个体。
从30例慢性牙周炎患者（18例男性和12例女性）和31例健康对照者（20例男性和11例女性）的白细胞中获取基因组DNA。受试者年龄在30至55岁之间，均为非吸烟者。使用聚合酶链反应（PCR），以白细胞介素-1β基因+3954位点两侧的特异性引物扩增DNA，并通过3%琼脂糖凝胶电泳进行分析。
采用卡方检验确定各组之间的基因型分布，并以95%置信区间估计相对风险。
慢性牙周炎组T等位基因的百分比更高，尽管差异无统计学意义。基因型之间的相对风险分析表明，与CC基因型相比，CT基因型的风险更高且差异具有统计学意义。总之，我们的数据表明，在印度南部人群中，白细胞介素-1β（+3954）基因多态性与慢性牙周炎之间无显著关联。
