Plastic Surgeon. Universidade de São Paulo - Medical School - São Paulo (SP), Brazil.
PhD. Universidade de São Paulo - Medical School -Microsurgery and Plastic Surgery Laboratory - São Paulo (SP), Brazil.
Acta Cir Bras. 2022 Aug 12;37(5):e370501. doi: 10.1590/acb370501. eCollection 2022.
To compare the effect of vein conduit filled with adipose tissue stem cells (ASC) on peripheral nerve injury regeneration.
We analyzed 30 male Wistar rats surgically submitted to a 5-mm gap on the sciatic nerve. Then, the animals were divided into three groups: nerve autografting (AG, n=10), autogenous inverted glycerol-conserved vein (VG, n=10), and autogenous inverted glycerol-conserved vein + ASC (VASCG, n=10). The study endpoints were neuromotor functional analysis, gastrocnemius muscle weight, and sciatic nerve graft histomorphometry analysis. In the histologic analysis, we added a control group (naïve nerve).
Regarding functional analysis (Walking tract- score), the findings at week 3 showed a difference between the AG and the VG (-96.6 vs. -59.6, p=0.01, respectively) and between the VG and the inverted vein + VASCG (-59.9 vs. -88.92, p=0.02). At week 12, this study showed a difference between the AG and the VG (-64.8 vs. -47.3, p=0.004, respectively), and also a difference between the VG and the VASCG (-47.3 vs. -57.4, p=0.02, respectively). There was no difference in the histomorphometry analysis (nerve diameter, Schwann cells counting). The gastrocnemius muscles on the intervention side were more atrophic when compared to the gastrocnemius muscles on the control side.
Our results suggested better functional recovery in the inverted vein group when compared to control group, and inverted vein + ASC group.
比较富含脂肪组织干细胞（ASC）的静脉管对周围神经损伤再生的影响。
我们分析了 30 只雄性 Wistar 大鼠，这些大鼠通过手术在坐骨神经上造成 5mm 的间隙。然后，将动物分为三组：神经自体移植（AG，n=10）、自体倒置甘油保存静脉（VG，n=10）和自体倒置甘油保存静脉+ ASC（VASCG，n=10）。研究终点为运动功能分析、腓肠肌重量和坐骨神经移植物组织形态计量学分析。在组织学分析中，我们增加了一个对照组（未处理神经）。
关于功能分析（行走轨迹评分），第 3 周时，AG 组与 VG 组之间存在差异（-96.6 与-59.6，p=0.01），VG 组与倒置静脉+VASCG 组之间也存在差异（-59.9 与-88.92，p=0.02）。第 12 周时，本研究显示 AG 组与 VG 组之间存在差异（-64.8 与-47.3，p=0.004），VG 组与 VASCG 组之间也存在差异（-47.3 与-57.4，p=0.02）。神经直径、施万细胞计数的组织形态计量学分析无差异。与对照组相比，干预侧的腓肠肌肌肉更加萎缩。
与对照组和倒置静脉+ASC 组相比，倒置静脉组的功能恢复更好。
