美洲大蠊乙醇提取物对慢性应激联合三硝基苯磺酸诱导的大鼠溃疡性结肠炎的改善作用。
The ethanol extract of Periplaneta Americana L. improves ulcerative colitis induced by a combination of chronic stress and TNBS in rats.
机构信息
MM. Dali University - The First Affiliated Hospital - Genetic Testing Center - Yunnan, China.
MM. Dali University - Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D - Yunnan, China.
出版信息
Acta Cir Bras. 2022 Aug 12;37(5):e370505. doi: 10.1590/acb370505. eCollection 2022.
PURPOSE
To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats.
METHODS
The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing.
RESULTS
Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota.
CONCLUSIONS
The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.
目的
研究美洲大蠊对慢性应激联合 2,4,6-三硝基苯磺酸灌肠诱导的溃疡性结肠炎（UC）的作用。
方法
采用慢性束缚应激、过度疲劳、不当饮食和 2,4,6-三硝基苯磺酸灌肠联合造模法建立 UC 大鼠模型，检测大鼠体重、疾病活动指数（DAI）、结肠黏膜损伤指数（CMDI）、组织病理学评分（HS）和促炎介质，采用酶联免疫吸附试验检测下丘脑促肾上腺皮质激素释放激素（CRH）或血浆促肾上腺皮质激素（ACTH）和皮质酮（CORT）含量，采用流式细胞术检测 T 淋巴细胞亚群比例，采用 16S rDNA 扩增子测序检测肠道微生物群。
结果
与 CS 联合 TNBS 诱导的 UC 大鼠相比，美洲大蠊治疗后体重减轻、DAI、CMDI、HS 和促炎介质均得到改善，同时观察到表皮生长因子（EGF）增加。此外，美洲大蠊还可降低 CRH 和 ACTH 的含量，调节脾中 CD3+、CD3+CD8+和 CD3+CD4+CD25+/CD4+的比值。比较而言，美洲大蠊改变了肠道微生物群的组成。
结论
美洲大蠊乙醇提取物可改善 CS 联合 TNBS 诱导的大鼠 UC。
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