Department of Zoology, Pachaiyappa's College for Men, Kanchipuram, 631 501, Tamil Nadu, India.
Department of Zoology, Nehru Memorial College, Puthanampatti, 621 007, Tamil Nadu, India.
Fish Shellfish Immunol. 2022 Sep;128:592-603. doi: 10.1016/j.fsi.2022.08.009. Epub 2022 Aug 14.
Individual and combined efficacy of chitooligosaccharides (COS) and alginic acid (AA) at 1 g, 2 g, and 3 g per kg diet was assessed on growth and disease resistance in silver carp (Hypophthalmichthys molitrix) against Edwardsiella ictaluri. Growth parameters including specific growth rate (SGR), weight gain (WG), and feed conversion rate (FCR) were significant in fish fed 2 g and 3 g kg of COS or AA, and fish fed combined COS + AA at 1, 2 and 3 kg diet. In all groups, the survival rate (SR) was recorded 100%, except in group fed 2 g kg AA diet. All the hematological and biochemical profiles significantly increased in groups fed 2 g and 3 g kg of COS, AA, and COS + AA diets. Lipase and amylase enzyme activities and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPx) antioxidant enzyme activities were significantly increased in fish fed 2 g and 3 g kg of COS, AA, and COS + AA diet. Respiratory burst (RB), lysozyme (Lyz), reactive oxygen species (ROS) activities, and immunoglobuline (Ig) level were enhanced significantly in fish fed 2 g kg of COS or COS + AA and all 3 g kg diets, whereas nitric acid (NO) production and serum AP activity were improved in 2 g kg COS + AA and 3 g kg COS or COS + AA diets. Pro-inflammatory cytokine such as IL-8 mRNA transcriptions was significant in 2 g kg COS + AA diet and all 3 g kg diet. The IL-10 anti-inflammatory cytokine mRNA transcriptions were significant in 3 g kg COS or COS + AA diets. This study was confirmed that H. molitrix fed with 3 g kg COS or COS + AA diets were better activity when compared to other diet.
壳寡糖（COS）和海藻酸钠（AA）在 1、2、3 g/kg 日粮中对草鱼（Hypophthalmichthys molitrix）爱德华氏菌的个体和联合功效进行了评估。在 2 g 和 3 g/kg 的 COS 或 AA 组中，生长参数（特定生长率[SGR]、增重[WG]和饲料转化率[FCR]）显著增加，在 1、2 和 3 kg 日粮中同时添加 COS+AA 的鱼也显著增加。在所有组中，除了 2 g/kg AA 日粮组外，存活率（SR）均为 100%。在 2 g 和 3 g/kg 的 COS、AA 和 COS+AA 日粮组中，所有的血液学和生化参数都显著增加。在 2 g 和 3 g/kg 的 COS、AA 和 COS+AA 日粮组中，脂肪酶和淀粉酶活性以及超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GPx）抗氧化酶活性均显著增加。在 2 g/kg 的 COS 或 COS+AA 组和所有 3 g/kg 的日粮组中，呼吸爆发（RB）、溶菌酶（Lyz）、活性氧（ROS）和免疫球蛋白（Ig）水平显著增强，而在 2 g/kg 的 COS+AA 和 3 g/kg 的 COS 或 COS+AA 日粮组中，硝酸（NO）产量和血清 AP 活性得到改善。在 2 g/kg 的 COS+AA 组和所有 3 g/kg 的日粮中，促炎细胞因子如白细胞介素 8（IL-8）mRNA 转录显著，而抗炎细胞因子白细胞介素 10（IL-10）mRNA 转录在 3 g/kg 的 COS 或 COS+AA 日粮中显著。本研究证实，与其他日粮相比，草鱼摄食 3 g/kg 的 COS 或 COS+AA 日粮时，活性更好。
