Department of Zoology, Pachaiyappa's College for Men, Kanchipuram, 631 501, Tamil Nadu, India.
Department of Zoology, Nehru Memorial College, Puthanampatti, 621 007, Tamil Nadu, India.
Fish Shellfish Immunol. 2023 Feb;133:108568. doi: 10.1016/j.fsi.2023.108568. Epub 2023 Jan 28.
The current study was designed to examine the impacts of dietary mannan-oligosaccharides (MOS) on growth, hemato-biochemical changes, digestive-antioxidant enzyme activity, immune response, and disease resistance of milkfish (Chanos chanos) fed diets contained MOS i.e. 1g, 2g, and 3g MOS. The growth parameters were significantly influence in milkfish fed all MOS diets, whereas the feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly influence with 2g or 3g MOS diets. The total protein (TP), globulin (GB), and glucose (GLU) levels, amylase, protease, liver enzymes were found significantly high in fish fed 2g or 3g MOS diets; but, lipase, trypsin, and alkaline phosphatase (ALP) enzymes were increased significantly at 3g MOS diet. All MOS inclusion levels were significantly increased total and Lactobacillus intestinal microflora population. The oxidative enzymes activity as superoxide desmutase (SOD) and catalyze (CAT) were progressively increased with all MOS supplementation diet, but the glutathione peroxidase (GPx) and lactate dehydrogenase (LDH) content were found significantly high in fish fed 2g or 3g MOS diets. Similarly, the reduced glutathione (GSH) and glutathione reductase (GR) contents were observed significantly high level in fish fed 3g MOS diet. The phagocytic (PC) and lysozyme (LYZ) activities were found gradually increase in fish fed increasing level of MOS diets, while the respiratory burst (RB) and malondialdehyde (MDA) activities were seen significant in fish fed 2g and 3g MOS diets. The current research work confirmed that C. chanos fed diets contained 3g kg MOS recorded better growth performance, digestive-antioxidant, immune response, and disease resistance.
本研究旨在探讨日粮甘露寡糖（MOS）对摄食 MOS 日粮的卵形鲳鲹（Chanos chanos）生长、血液生化变化、消化-抗氧化酶活性、免疫反应和抗病力的影响。MOS 日粮对卵形鲳鲹的生长参数有显著影响，而摄食 2g 或 3g MOS 日粮时，饲料转化率（FCR）和蛋白质效率比（PER）有显著影响。摄食 2g 或 3g MOS 日粮的鱼总蛋白（TP）、球蛋白（GB）和葡萄糖（GLU）水平、淀粉酶、蛋白酶、肝酶显著升高；但脂肪酶、胰蛋白酶和碱性磷酸酶（ALP）在 3g MOS 日粮中显著增加。所有 MOS 水平均显著增加了肠道总菌和乳酸菌的数量。超氧化物歧化酶（SOD）和过氧化氢酶（CAT）等氧化酶活性随 MOS 补充量的增加而逐渐增加，但 2g 或 3g MOS 日粮组的谷胱甘肽过氧化物酶（GPx）和乳酸脱氢酶（LDH）含量显著升高。同样，摄食 3g MOS 日粮的鱼的还原型谷胱甘肽（GSH）和谷胱甘肽还原酶（GR）含量也显著升高。摄食 MOS 水平逐渐升高的鱼的吞噬（PC）和溶菌酶（LYZ）活性逐渐增加，而摄食 2g 和 3g MOS 日粮的鱼的呼吸爆发（RB）和丙二醛（MDA）活性显著增加。本研究工作证实，摄食含 3g kg MOS 日粮的卵形鲳鲹生长性能、消化-抗氧化、免疫反应和抗病力较好。
