Maslennikov Roman, Ivashkin Vladimir, Alieva Aliya, Poluektova Elena, Kudryavtseva Anna, Krasnov George, Zharkova Maria, Zharikov Yuri
Department of Internal Medicine, Gastroenterology and Hepatology, Sechenov University, Moscow 119435, Russia.
Scientific Community for Human Microbiome Research, Moscow 119435, Russia.
World J Hepatol. 2022 Jun 27;14(6):1210-1225. doi: 10.4254/wjh.v14.i6.1210.
Gut dysbiosis and changes in body composition (, a decrease in the proportion of muscle mass and an increase in extracellular fluid) are common in cirrhosis.
To study the relationship between the gut microbiota and body composition in cirrhosis.
This observational study included 46 patients with cirrhosis. Stool microbiome was assessed using 16S rRNA gene sequencing. Multifrequency bioelectrical impedance analysis was performed to assess body composition in these patients.
An increase in fat mass and a decrease in body cell mass were noted in 23/46 (50.0%) and 15/46 (32.6%) patients, respectively. Changes in the gut microbiome were not independently associated with the fat mass percentage in cirrhosis. The abundance of ( = 0.041) and ( = 0.001) increased, whereas that of ( = 0.006), ( = 0.021), ( = 0.033), ( = 0.043), ( = 0.028), and ( = 0.015) decreased in the gut microbiome of patients with body cell mass deficiency. The amount of extracellular fluid increased in 22/46 (47.6%) patients. Proteobacteria abundance ( < 0.001) increased, whereas Firmicutes ( = 0.023), Actinobacteria ( = 0.026), Bacilli ( = 0.008), ( = 0.027), ( = 0.038), ( = 0.047), ( = 0.015), ( = 0.003), ( = 0.024), ( = 0.002), ( = 0.030), ( = 0.040), ( = 0.023), ( = 0.008), and ( = 0.024) abundance decreased in these patients. Patients with clinically significant ascites ( = 9) had a higher abundance of Proteobacteria ( = 0.031) and a lower abundance of Actinobacteria ( = 0.019) and Bacteroidetes ( = 0.046) than patients without clinically significant ascites ( = 37).
Changes in the amount of body cell mass and extracellular fluid are associated with changes in the gut microbiome in cirrhosis patients.
肠道菌群失调和身体成分变化（即肌肉量比例降低和细胞外液增加）在肝硬化患者中很常见。
研究肝硬化患者肠道微生物群与身体成分之间的关系。
这项观察性研究纳入了46例肝硬化患者。使用16S rRNA基因测序评估粪便微生物组。对这些患者进行多频生物电阻抗分析以评估身体成分。
分别有23/46（50.0%）和15/46（32.6%）的患者出现脂肪量增加和身体细胞量减少。肠道微生物组的变化与肝硬化患者的脂肪量百分比无独立相关性。身体细胞量缺乏患者的肠道微生物组中，[具体菌种1]（P = 0.041）和[具体菌种2]（P = 0.001）丰度增加，而[具体菌种3]（P = 0.006）、[具体菌种4]（P = 0.021）、[具体菌种5]（P = 0.033）、[具体菌种6]（P = 0.043）、[具体菌种7]（P = 0.028）和[具体菌种8]（P = 0.015）丰度降低。22/46（47.6%）的患者细胞外液量增加。这些患者中变形菌门丰度（P < 0.001）增加，而厚壁菌门（P = 0.023）、放线菌门（P = 0.026）、芽孢杆菌属（P = 0.008）、[具体菌种9]（P = 0.027）、[具体菌种10]（P = 0.038）、[具体菌种11]（P = 0.047）、[具体菌种12]（P = 0.015）、[具体菌种13]（P = 0.003）、[具体菌种14]（P = 0.024）、[具体菌种15]（P = 0.002）、[具体菌种16]（P = 0.030）、[具体菌种17]（P = 0.040）、[具体菌种18]（P = 0.023）、[具体菌种19]（P = 0.008）和[具体菌种20]（P = 0.024）丰度降低。有临床显著性腹水的患者（n = 9）比无临床显著性腹水的患者（n = 37）具有更高的变形菌门丰度（P = 0.031）和更低的放线菌门（P = 0.019）及拟杆菌门（P = 0.046）丰度。
肝硬化患者身体细胞量和细胞外液量的变化与肠道微生物组的变化相关。
