Development of inclusion complexes of 2-hydroxypropyl-β-cyclodextrin with L. essential oil by freeze-drying and kneading methods for application as L. larvicide.

Inclusion complexes (ICs) of 2-hydroxypropyl-β-cyclodextrin with the essential oil (EO) from Seculo XXI cultivar of were prepared using kneading (KN) and freeze-drying (FD) methods. The resulting ICs clusters have a nanometric size, with a diameter of approximately 80 and 40 nm for KN and FD, respectively. Complexation efficiency was 80.3% and 50.8% for KN and FD methods, respectively. The larvicidal activity of the EO in DMSO on had LC and LC values of 51.49 and 64.51 µg mL, respectively. For the KN method, the toxicity corresponded to 77.54 and 107.29 µg mL for LC and LC, respectively. FD method demonstrated toxicity at concentrations above 600 µg mL. Thus, ICs enable the use of EO in breeding sites for , thus being potential products to be commercially exploited.