Cheng Sen-Sung, Chang Hui-Ting, Chang Shang-Tzen, Tsai Kun-Hsien, Chen Wei-June
Department of Forestry, National Taiwan University, Taipei 106, Taiwan.
Bioresour Technol. 2003 Aug;89(1):99-102. doi: 10.1016/s0960-8524(03)00008-7.
The bioactivity of 14 essential oils from five plants has been studied using the brine shrimp lethality test and the Aedes aegypti larvicidal assay. All essential oils screened had LC50 values smaller than 200 microg/ml, showing significant lethality against brine shrimp. In addition, nine of the 14 essential oils tested showed toxicity against the fourth-instar A. aegypti larvae in 24 h (LC50<100 microg/ml). Of these, the leaf and bark essential oils of Cryptomeria japonica demonstrated high larvicidal activity, the most active being the leaf essential oil of C. japonica, with a LC50=37.6 microg/ml (LC90=71.9 microg/ml), followed by the bark essential oil of C. japonica also showing high activity against A. aegypti larvae, with a LC50=48.1 microg/ml (LC90=130.3 microg/ml). The results obtained from this study suggest that the leaf and bark essential oils of C. japonica are promising as larvicides against A. aegypti larvae and could be useful in the search for new natural larvicidal compounds.
利用卤虫致死试验和埃及伊蚊幼虫杀灭试验，对来自五种植物的14种精油的生物活性进行了研究。所有筛选的精油的半数致死浓度（LC50）值均小于200微克/毫升，对卤虫显示出显著的致死性。此外，所测试的14种精油中有9种在24小时内对埃及伊蚊四龄幼虫显示出毒性（LC50<100微克/毫升）。其中，日本柳杉的叶精油和树皮精油表现出较高的杀幼虫活性，最具活性的是日本柳杉叶精油，LC50 = 37.6微克/毫升（LC90 = 71.9微克/毫升），其次是日本柳杉树皮精油，对埃及伊蚊幼虫也表现出高活性，LC50 = 48.1微克/毫升（LC90 = 130.3微克/毫升）。本研究所得结果表明，日本柳杉的叶精油和树皮精油有望作为针对埃及伊蚊幼虫的杀幼虫剂，并且在寻找新的天然杀幼虫化合物方面可能有用。
