College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
Analyst. 2022 Sep 12;147(18):4073-4081. doi: 10.1039/d2an00993e.
The achievement of high-throughput separation and high-sensitivity detection of complex samples has been one of the most challenging issues in the field of analytical science. The application of a single technology alone could not satisfy the above requirements. The combination of technologies with the capability of high-efficiency separation and high-sensitivity structural-recognition is highly desired to meet the technical requirements. Herein, an automatic high-performance liquid chromatography (HPLC)-surface enhanced Raman spectroscopy (SERS) hyphenated system using paper substrates as the "interface" was constructed to achieve efficient separation and real-time detection. A homogeneous Au nanoparticle was printed on the hydrophobic filter paper with the inkjet technology. The prepared substrates served as a linkage for the continuous realization of HPLC and SERS functions. The complex system was separated by HPLC, and the effluents were loaded onto automatically and continuously replaceable paper substrates for real time SERS measurements. The continuous rapid separation and real-time detection of various two-component mixtures were achieved with the separation efficiency and detection sensitivity of each technology. The results demonstrated that the HPLC-SERS hyphenated system exhibited the complementary capability of the on-line separation and continuous structural identification of illegal additives in real samples. The detection sensitivity was increased by an order of magnitude to reach 10 mol dm, and the efficiency and accuracy for the separation and identification on the multi-components samples were higher than those of the individual HPLC or SERS technology. It is believed that the continuous paper substrate-based HPLC-SERS hyphenated system would be developed as a promising technique for the separation and identification of multi-components mixtures with high throughput.
实现复杂样品的高通量分离和高灵敏度检测一直是分析科学领域最具挑战性的问题之一。仅应用单一技术无法满足上述要求。需要结合具有高效分离和高灵敏度结构识别能力的技术，以满足技术要求。在此，构建了一种自动高效液相色谱（HPLC）-表面增强拉曼光谱（SERS）联用系统，该系统使用纸基作为“接口”，以实现高效分离和实时检测。通过喷墨技术将均一的金纳米粒子打印在疏水性滤纸上。制备的基底作为连续实现 HPLC 和 SERS 功能的连接体。通过 HPLC 对复杂体系进行分离，将流出物加载到自动且可连续更换的纸基上，以进行实时 SERS 测量。通过每种技术的分离效率和检测灵敏度，实现了各种二组分混合物的连续快速分离和实时检测。结果表明，HPLC-SERS 联用系统表现出在线分离和实时检测真实样品中非法添加剂的连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到 10 mol dm，并且在多组分样品的分离和鉴定方面的效率和准确性均高于单独的 HPLC 或 SERS 技术。相信基于连续纸基的 HPLC-SERS 联用系统将作为高通量分离和鉴定多组分混合物的有前途的技术得到发展。
