Key Laboratory of depression animal model based on TCM syndrome, Jiangxi Administration of Traditional Chinese Medicine, Key Laboratory of TCM for prevention and treatment of brain diseases with cognitive impairment, Jiangxi Province, Jiangxi University of Chinese Medicine, 1688 Meiling Road, Nanchang, 330006, China.
Jiahehongsheng (Shenzhen) Health Industry Group, 2028 Shenyan Road, Haishan street, 518081, Shenzhen, China.
Chem Biodivers. 2022 Sep;19(9):e202100961. doi: 10.1002/cbdv.202100961. Epub 2022 Aug 31.
Herba Patriniae (HP) is widely used as a medicinal and edible material in China. Besides food value, HP attracts more attention due to its medicinal potential. Patrinia villosa Juss. (PV) and Patrinia scabiosaefolia Fisch. (PS) are the two species origins of HP. These two of HP show different effects on cell proliferation, migration, angiogenesis and anti-diabetic. As we have previously reported, PV and PS show significant differences on their anti-inflammatory ability in the same experimental model. Comparing the ingredient profiles of two different sources will not only facilitate the understanding of their medicinal effects, but also help the development and research of new activities. However, still now, there is no systematic and detailed study to compare the components of PV and PS. In present study, ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was employed to achieve a high-throughput qualitative and thorough analysis of the chemical composition spectrum of HP. A total of 164 compounds were identified, among these compounds, 127 compounds were identified from PV, and 107 compounds were identified from PS. Most of the chemical components was discovered for the first time. Flavonoids, saponins, terpenoids and organic acids, as the main ingredients in PV and PS were 45.45 %vs 28.46 %, 12.61 % vs. 32.09 %, 14.33 % vs. 22.38 % and 14.58 % vs. 6.79 %, respectively. Flavonoids are the main components of PV, while PS is rich in saponins. PV and PS were classified into two groups by principal component analysis (PCA) and screened out the main molecular differences responsible by orthogonal partial least squares discriminant analysis (OPLS-DA). All the results will be a guide for the quality control, functional activity research, or better clinic use based on the ingredients profile between these two species. Besides, this first study on ingredients profile of two species origins will be beneficial for potential and best resources utilization of both PV and PS.
夏枯草（HP）在中国被广泛用作药用和食用材料。除了食用价值外，由于其药用潜力，HP 也引起了更多的关注。夏枯草（PV）和狭基线纹香茶菜（PS）是 HP 的两个物种来源。这两种 HP 在细胞增殖、迁移、血管生成和抗糖尿病方面表现出不同的作用。正如我们之前报道的，在相同的实验模型中，PV 和 PS 在抗炎能力上表现出显著差异。比较两种不同来源的成分谱不仅有助于了解它们的药用效果，还有助于新活性的开发和研究。然而，到目前为止，还没有系统和详细的研究来比较 PV 和 PS 的成分。在本研究中，采用超高效液相色谱-四极杆飞行时间质谱联用技术对 HP 的化学成分谱进行高通量定性和全面分析。共鉴定出 164 种化合物，其中 127 种化合物从 PV 中鉴定出来，107 种化合物从 PS 中鉴定出来。大多数化学成分都是首次发现。黄酮类、皂苷类、萜类和有机酸是 PV 和 PS 的主要成分，分别为 45.45%vs28.46%、12.61%vs32.09%、14.33%vs22.38%和 14.58%vs6.79%。黄酮类化合物是 PV 的主要成分，而 PS 富含皂苷。通过主成分分析（PCA）将 PV 和 PS 分为两组，并通过正交偏最小二乘判别分析（OPLS-DA）筛选出主要的分子差异。所有结果将为基于这两种植物的成分谱进行质量控制、功能活性研究或更好的临床应用提供指导。此外，对这两个物种起源的成分谱的首次研究将有利于 PV 和 PS 的潜在和最佳资源利用。
